Document Detail


Colony-blot assay with anti-p60 antibodies as a method for quick identification of Listeria in food.
MedLine Citation:
PMID:  11843414     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study evaluated the ability to isolate Listeria from foods, using shortened procedure of sample enrichment followed by immunomagnetic separation or filtration methods, and serological identification of isolated bacteria by colony-blot and Western blot methods with anti-p60 antibodies. By these rapid methods, identification of Listeria was achieved in much shorter time (40-48 h) than with standard cultivation and biochemical identification procedures. The rapid methods used are easy to perform and, what is most important, their specificity is very high and fulfills the expectations. The possibility to select Listeria colonies growing on non-selective media by blotting with anti-p60 antiserum seems to be particularly valuable in examination of food samples containing/not too many Listeria (1-10 CFU/25 g). However, the blot method using anti-PepD mAb specific to unique region of L. monocytogenes p60 is necessary to distinguish L. monocytogenes from other Listeria species.
Authors:
Marzena Wieckowska-Szakiel; Andreas Bubert; Marek Rózalski; Urszula Krajewska; Wiesława Rudnicka; Barbara Rózalska
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  72     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-02-14     Completed Date:  2002-06-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  63-71     Citation Subset:  IM    
Affiliation:
Department of Infectious Biology, Institute of Microbiology and Immunology, University of Lódź, Poland.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial / immunology*
Antibody Specificity
Bacterial Proteins / immunology*
Blotting, Western / methods
Colony Count, Microbial
Food Contamination
Food Microbiology*
Immunomagnetic Separation / methods
Listeria / immunology,  isolation & purification*
Sensitivity and Specificity
Time Factors
Chemical
Reg. No./Substance:
0/60 kDa protein, Listeria monocytogenes; 0/Antibodies, Bacterial; 0/Bacterial Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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