Document Detail


Colony Forming Efficiency and microscopy analysis of multi-wall carbon nanotubes cell interaction.
MedLine Citation:
PMID:  20435104     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this work, we present a complete physicochemical characterization of multi-wall carbon nanotubes (mwCNTs) in order to assess their potential toxicological effects in in vitro cell models using Colony Forming Efficiency (CFE) assay. We verified that Dimethyl Sulfoxide (DMSO) was a more suitable solvent to disperse mwCNTs compared to culture medium guaranteeing reproducibility in the preparation of testing dilutions. The CFE assay was carried out on five mammalian cell lines representing the potentially exposed and/or target organs for nanomaterials (lung, liver, kidney, intestine, skin), as well as on mouse fibroblasts cell line, which usually is considered a sensitive model to verify in vitro cytotoxicity of test compounds. A statistically significant toxic effect was found only in human alveolar basal epithelial cells and immortalized mouse fibroblasts, for which the interaction between mwCNTs and cells was additionally studied by Atomic Force and Scanning Electron Microscopy. In this study, we considered and suggested the CFE assay as a promising test for screening studies of cytotoxicity. In addition, combining in vitro tests with physicochemical analysis, this work underlines basic points to be considered when research on nanomaterials has to be carried out, to set up, in our opinion, well-defined and suitable experimental planning and procedures.
Authors:
Jessica Ponti; Renato Colognato; Hubert Rauscher; Sabrina Gioria; Francesca Broggi; Fabio Franchini; Cesar Pascual; Guido Giudetti; François Rossi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-29
Journal Detail:
Title:  Toxicology letters     Volume:  197     ISSN:  1879-3169     ISO Abbreviation:  Toxicol. Lett.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7709027     Medline TA:  Toxicol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  29-37     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
European Commission, Joint Research Centre, Institute for Health and Consumer Protection, Nanobiosciences Unit, 21027 Ispra, VA, Italy. jessica.ponti@jrc.ec.europa.eu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Colony-Forming Units Assay*
Humans
Kinetics
Mice
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Nanotubes, Carbon / toxicity*,  ultrastructure
Toxicity Tests / methods*
Chemical
Reg. No./Substance:
0/Nanotubes, Carbon

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