Document Detail

Colonization and osteogenic differentiation of different stem cell sources on electrospun nanofiber meshes.
MedLine Citation:
PMID:  20504075     Owner:  NLM     Status:  MEDLINE    
Numerous challenges remain in the successful clinical translation of cell-based therapies for musculoskeletal tissue repair, including the identification of an appropriate cell source and a viable cell delivery system. The aim of this study was to investigate the attachment, colonization, and osteogenic differentiation of two stem cell types, human mesenchymal stem cells (hMSCs) and human amniotic fluid stem (hAFS) cells, on electrospun nanofiber meshes. We demonstrate that nanofiber meshes are able to support these cell functions robustly, with both cell types demonstrating strong osteogenic potential. Differences in the kinetics of osteogenic differentiation were observed between hMSCs and hAFS cells, with the hAFS cells displaying a delayed alkaline phosphatase peak, but elevated mineral deposition, compared to hMSCs. We also compared the cell behavior on nanofiber meshes to that on tissue culture plastic, and observed that there is delayed initial attachment and proliferation on meshes, but enhanced mineralization at a later time point. Finally, cell-seeded nanofiber meshes were found to be effective in colonizing three-dimensional scaffolds in an in vitro system. This study provides support for the use of the nanofiber mesh as a model surface for cell culture in vitro, and a cell delivery vehicle for the repair of bone defects in vivo.
Yash M Kolambkar; Alexandra Peister; Andrew K Ekaputra; Dietmar W Hutmacher; Robert E Guldberg
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  16     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2011-01-20     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3219-30     Citation Subset:  IM    
Wallace H. Coulter Department of Biomedical Engineering, Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
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MeSH Terms
Alkaline Phosphatase / metabolism
Amniotic Fluid / cytology*
Calcium / metabolism
Cell Differentiation / physiology
Cell Survival / physiology
Mesenchymal Stromal Cells / cytology*,  metabolism
Osteogenesis / physiology
Spectroscopy, Fourier Transform Infrared
Stem Cells / cytology*,  metabolism
Tissue Engineering / methods
Grant Support
Reg. No./Substance:
7440-70-2/Calcium; EC Phosphatase

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