Document Detail


Colonization of Aspergillus japonicus on synthetic materials and application to the production of fructooligosaccharides.
MedLine Citation:
PMID:  19251252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ability of Aspergillus japonicus ATCC 20236 to colonize different synthetic materials (polyurethane foam, stainless steel sponge, vegetal fiber, pumice stones, zeolites, and foam glass) and to produce fructooligosaccharides (FOS) from sucrose (165 g/L) is described. Cells were immobilized in situ by absorption, through direct contact with the carrier particles at the beginning of fermentation. Vegetal fiber was the best immobilization carrier as A. japonicus grew well on it (1.25 g/g carrier), producing 116.3g/L FOS (56.3g/L 1-kestose, 46.9 g/L 1-nystose, and 13.1g/L 1-beta-fructofuranosyl nystose) with 69% yield (78% based only in the consumed sucrose amount), giving also elevated activity of the beta-fructofuranosidase enzyme (42.9 U/mL). In addition, no loss of material integrity, over a 2 day-period, was found. The fungus also immobilized well on stainless steel sponge (1.13 g/g carrier), but in lesser extents on polyurethane foam, zeolites, and pumice stones (0.48, 0.19, and 0.13 g/g carrier, respectively), while on foam glass no cell adhesion was observed. When compared with the FOS and beta-fructofuranosidase production by free A. japonicus, the results achieved using cells immobilized on vegetal fiber were closely similar. It was thus concluded that A. japonicus immobilized on vegetal fiber is a potential alternative for high production of FOS at industrial scale.
Authors:
Solange I Mussatto; Cristóbal N Aguilar; Lígia R Rodrigues; José A Teixeira
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-04
Journal Detail:
Title:  Carbohydrate research     Volume:  344     ISSN:  1873-426X     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-04-10     Completed Date:  2009-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  795-800     Citation Subset:  IM    
Affiliation:
IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal. solange@deb.uminho.pt
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MeSH Terms
Descriptor/Qualifier:
Aspergillus / enzymology,  growth & development*,  metabolism*
Fructose / analogs & derivatives*
Oligosaccharides / biosynthesis*
Sucrose / metabolism
beta-Fructofuranosidase / metabolism
Chemical
Reg. No./Substance:
0/Oligosaccharides; 30237-26-4/Fructose; 57-50-1/Sucrose; EC 3.2.1.26/beta-Fructofuranosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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