Document Detail


Colonic mucosal proliferation is related to serum deoxycholic acid levels.
MedLine Citation:
PMID:  10223558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Hyperproliferation of the colorectal mucosa is regarded as an early step in colorectal carcinogenesis. Deoxycholic acid, a secondary bile acid, stimulates colorectal epithelial proliferation in animals and is considered a tumor promoter in human colorectal carcinogenesis. The aim of this study was to investigate the correlation between colorectal mucosal proliferation and the serum deoxycholic acid level. METHODS: From each of 19 patients (10 men and 9 women) with (n = 3) or without (n = 16) colorectal adenoma, 18 biopsy specimens were obtained by colonoscopy, 3 from each of the 6 colonic segments. A crude nuclei fraction was prepared, and DNA was stained by propidium iodide to determine the proliferative index (the percentage of cells in the S and G2/M phases of the cell cycle) by flow cytometry. Serum levels of deoxycholic acid were determined by gas-liquid chromatography. RESULTS: The colonic proliferation rates (median of the values obtained in all segments, 14.1%; range, 10.0-18.7%) and the fasting serum deoxycholic acid levels (median, 0.86 micromol/L; range, 0.28-1.58 micromol/L) showed a significant correlation (r = 0.51, P = 0.03). Serum lithocholic, cholic, chenodeoxycholic, and ursodeoxycholic acid levels were not correlated with the proliferation rates. CONCLUSIONS: Levels of deoxycholic acid in serum are correlated with the rates of the colorectal mucosa. These results are consistent with the concept that deoxycholic acid promotes colorectal carcinogenesis.
Authors:
T Ochsenkühn; E Bayerdörffer; A Meining; M Schinkel; C Thiede; V Nüssler; M Sackmann; R Hatz; A Neubauer; G Paumgartner
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer     Volume:  85     ISSN:  0008-543X     ISO Abbreviation:  Cancer     Publication Date:  1999 Apr 
Date Detail:
Created Date:  1999-05-13     Completed Date:  1999-05-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374236     Medline TA:  Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1664-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine II, Klinikum Grosshadern, University of Munich, Germany.
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MeSH Terms
Descriptor/Qualifier:
Adenoma / blood,  pathology*
Adult
Bile Acids and Salts / blood
Biological Markers
Carcinogens / analysis*
Cell Division
Colon / cytology*,  pathology
Colonic Neoplasms / blood,  pathology*
Colonoscopy
Deoxycholic Acid / blood*
Female
Humans
Intestinal Mucosa / cytology*,  pathology
Male
Mitotic Index
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Biological Markers; 0/Carcinogens; 83-44-3/Deoxycholic Acid

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