| Colonic expression of c-myb is initiated in utero and continues throughout adult life. | |
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MedLine Citation:
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PMID: 8809414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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c-myb expression is an indicator of hemopoietic cell proliferation and its down-regulation occurs as an early event in cellular differentiation in vitro. We have previously demonstrated c-Myb protein expression in normal adult colon and tumor-derived colonic cell lines and have also shown that the proliferation of colon carcinoma cell lines is c-myb dependent. This study used the techniques of RNase protection and immunohistochemistry to define c-myb mRNA and protein expression in the normal adult mouse and mouse embryos, with special focus on the colon. These experiments revealed wide-spread c-myb mRNA expression in mouse embryos including nonhemopoietic tissues, and developmental time course studies revealed alterations in organ-specific c-myb mRNA expression. Immunohistochemical studies confirmed the embryonal expression of c-Myb in concordance with the mRNA data as well as c-Myb expression throughout the length of the adult mouse colonic crypt. In contrast, staining for the proliferating cell nuclear antigen was confined to the lower one third of the crypt. In addition, c-Myb staining extended beyond that of the proliferating cell nuclear antigen within the germinal centers of the spleen. These data suggest that c-myb: (a) has a role in the embryonic development of nonhemopoietic organs; (b) plays a specific role in the biology of the normal adult colonic crypt; and (c) expression is not inextricably linked to proliferation in vivo. |
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Authors:
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M A Rosenthal; M A Thompson; S Ellis; R H Whitehead; R G Ramsay |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research Volume: 7 ISSN: 1044-9523 ISO Abbreviation: Cell Growth Differ. Publication Date: 1996 Jul |
Date Detail:
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Created Date: 1997-04-29 Completed Date: 1997-04-29 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9100024 Medline TA: Cell Growth Differ Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 961-7 Citation Subset: IM |
Affiliation:
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Ludwig Institute for Cancer Research, Tumor Biology Branch, Melbourne, Victoria, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Colon / embryology, metabolism* Female Gene Expression Regulation, Developmental* Immunohistochemistry Mice Pregnancy Proto-Oncogene Proteins / biosynthesis*, genetics Proto-Oncogene Proteins c-myb RNA, Messenger / biosynthesis Trans-Activators / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/RNA, Messenger; 0/Trans-Activators |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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