Document Detail

Colonial morphology of treponemes observed by electron microscopy.
MedLine Citation:
PMID:  6727709     Owner:  NLM     Status:  MEDLINE    
The colonial morphology of three strains of cultivable, nonpathogenic treponemes including a human oral treponeme was examined by light and electron microscopy. Treponema phagedenis strains Kazan and Reiter produced large white colonies on the surface of solid media composed of sterility test broth, 0.9 to 3.1% agar, rifampin, and 12.5% rabbit or horse serum. A human oral treponeme, strain G7201 , grew as diffused white zones on 0.9 to 3.1% agar plates. Under the cultural conditions employed agar concentrations slightly affected the time of appearance of colonies of the three strains of treponemes. When the colonies of these three strains were viewed by scanning electron microscopy, differences in their colonial morphology were observed. The 11-day-old colonies of human oral strain G7201 were very small, 5 to 15 micron in diameter, and had a slight irregular border. Kazan treponemes developed circular, entire and low convex colonies. Scanning and transmission electron microscopy revealed that the colonies of Reiter treponemes contained spherical forms almost up to 5 micron in diameter, each consisting of an outer membrane and a treponemal main body. They were very similar to the spherical bodies produced by strain G7201 in sucrose-containing broth.
T Umemoto; I Namikawa; M Yamamoto
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Microbiology and immunology     Volume:  28     ISSN:  0385-5600     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  1984  
Date Detail:
Created Date:  1984-07-09     Completed Date:  1984-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  11-22     Citation Subset:  IM    
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MeSH Terms
Culture Media
Microscopy, Electron
Microscopy, Electron, Scanning
Treponema / cytology*,  isolation & purification,  ultrastructure
Reg. No./Substance:
0/Culture Media; 9002-18-0/Agar

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