| Colocalization of SCD1 and DGAT2: implying preference for endogenous monounsaturated fatty acids in triglyceride synthesis. | |
| | |
MedLine Citation:
|
PMID: 16751624 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Stearoyl-coenzyme A desaturase (SCD) is an endoplasmic reticulum (ER) protein that catalyzes the Delta9-cis desaturation of saturated fatty acids. Mice with targeted disruption in SCD1 (Scd1(-/-)) have significant reduction in the tissue content of triglycerides, suggesting that monounsaturated fatty acids endogenously synthesized by SCD1 are important for triglyceride synthesis. Acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) is the enzyme that catalyzes the final reaction in the synthesis of triglycerides. The lack of DGAT2, one of the two DGAT isoforms, results in almost a complete loss of tissue triglycerides. We hypothesize that SCD1 participates in triglyceride synthesis by providing a more accessible pool of monounsaturated fatty acids through substrate channeling. In this study, we test whether SCD1 is proximal to DGAT2 by colocalization study with confocal microscopy, coimmunoprecipitation, and fluorescence resonance energy transfer using HeLa cells as the model of study. All of the results suggest that SCD1 and DGAT2 are located very close to each other in the ER, which is a very important criterion for the channeling of substrate. By performing subcellular fractionation using mouse livers, we also show, for the first time, that SCD is present in the mitochondria-associated membrane. |
| | |
Authors:
|
Weng Chi Man; Makoto Miyazaki; Kiki Chu; James Ntambi |
Related Documents
:
|
3897564 - Sonographic appearance of normal lymph nodes. 16658264 - Polar indole-3-acetic acid diffusion in nonliving and model systems. 6612094 - Lipogenic capacity and relative contribution of the different tissues and organs to lip... 6368714 - Radioisotopic method for the measurement of lipolysis in small samples of human adipose... 20618724 - Effect of folic acid fortification on the incidence of neural tube defects. 15762174 - Approaches for functional characterization of diatom silicic acid transporters. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2006-06-02 |
Journal Detail:
|
Title: Journal of lipid research Volume: 47 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2006 Sep |
Date Detail:
|
Created Date: 2006-08-22 Completed Date: 2006-10-04 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
|
Languages: eng Pagination: 1928-39 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Western / methods Diacylglycerol O-Acyltransferase / genetics, metabolism* Fatty Acids, Monounsaturated / metabolism* Flow Cytometry / methods Fluorescence Resonance Energy Transfer / methods Hela Cells Humans Immunoprecipitation / methods Mice Mice, Inbred Strains Microscopy, Confocal / methods Mitochondrial Membranes / metabolism Models, Biological Recombinant Fusion Proteins / genetics, metabolism Stearoyl-CoA Desaturase / genetics, metabolism* Triglycerides / biosynthesis* |
| Grant Support | |
ID/Acronym/Agency:
|
R01DK-62388/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Fatty Acids, Monounsaturated; 0/Recombinant Fusion Proteins; 0/Triglycerides; EC 1.14.19.1/Stearoyl-CoA Desaturase; EC 2.3.1.20/Diacylglycerol O-Acyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Adipose tissue-specific CETP expression in mice: impact on plasma lipoprotein metabolism.
Next Document: Crystal structure and mutational analysis of the DaaE adhesin of Escherichia coli.