Document Detail


Colocalization of SCD1 and DGAT2: implying preference for endogenous monounsaturated fatty acids in triglyceride synthesis.
MedLine Citation:
PMID:  16751624     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Stearoyl-coenzyme A desaturase (SCD) is an endoplasmic reticulum (ER) protein that catalyzes the Delta9-cis desaturation of saturated fatty acids. Mice with targeted disruption in SCD1 (Scd1(-/-)) have significant reduction in the tissue content of triglycerides, suggesting that monounsaturated fatty acids endogenously synthesized by SCD1 are important for triglyceride synthesis. Acyl-coenzyme A:diacylglycerol acyltransferase (DGAT) is the enzyme that catalyzes the final reaction in the synthesis of triglycerides. The lack of DGAT2, one of the two DGAT isoforms, results in almost a complete loss of tissue triglycerides. We hypothesize that SCD1 participates in triglyceride synthesis by providing a more accessible pool of monounsaturated fatty acids through substrate channeling. In this study, we test whether SCD1 is proximal to DGAT2 by colocalization study with confocal microscopy, coimmunoprecipitation, and fluorescence resonance energy transfer using HeLa cells as the model of study. All of the results suggest that SCD1 and DGAT2 are located very close to each other in the ER, which is a very important criterion for the channeling of substrate. By performing subcellular fractionation using mouse livers, we also show, for the first time, that SCD is present in the mitochondria-associated membrane.
Authors:
Weng Chi Man; Makoto Miyazaki; Kiki Chu; James Ntambi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-06-02
Journal Detail:
Title:  Journal of lipid research     Volume:  47     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-22     Completed Date:  2006-10-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1928-39     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western / methods
Diacylglycerol O-Acyltransferase / genetics,  metabolism*
Fatty Acids, Monounsaturated / metabolism*
Flow Cytometry / methods
Fluorescence Resonance Energy Transfer / methods
Hela Cells
Humans
Immunoprecipitation / methods
Mice
Mice, Inbred Strains
Microscopy, Confocal / methods
Mitochondrial Membranes / metabolism
Models, Biological
Recombinant Fusion Proteins / genetics,  metabolism
Stearoyl-CoA Desaturase / genetics,  metabolism*
Triglycerides / biosynthesis*
Grant Support
ID/Acronym/Agency:
R01DK-62388/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids, Monounsaturated; 0/Recombinant Fusion Proteins; 0/Triglycerides; EC 1.14.19.1/Stearoyl-CoA Desaturase; EC 2.3.1.20/Diacylglycerol O-Acyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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