Document Detail


Colocalization of GH, TSH and prolactin, but not ACTH, with betaLH-immunoreactivity: evidence for pluripotential cells in the ovine pituitary.
MedLine Citation:
PMID:  15647919     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Increasing evidence suggests that multihormonal cells in the pituitary gland may be more commonplace than previously thought. This has forced us to reconsider our classical view of cell populations in the pituitary gland. Studies so far have focused almost exclusively on the rat, and there is a dearth of information on other species. Our first objective was to determine whether a subpopulation of gonadotropes also express somatotropin in the ewe, as reported in the rat. In addition, we sought to determine whether gonadotropes express any of the other known pituitary hormones. Finally, we investigated whether the stage of the estrous cycle influenced the occurrence of these pluripotential gonadotropes. We found that a small population of betaLH-immunoreactive cells also expresses immunoreactive GH, prolactin and TSH. No gonadotropes colocalized with ACTH. Significantly (P<0.001) more gonadotropes expressed GH during the luteal (10.7+/-0.4%) than the late follicular (5.4+/-0.3%) phase but there was no difference between the luteal and follicular phases in the proportion of gonadotropes expressing prolactin (follicular: 5.7+/-0.7%; luteal: 5.5+/-0.6%) or TSH (follicular: 3.1+/-0.7%; luteal: 4.2+/-0.5%). Similarly, there was a significant (P<0.05) difference in the proportion of GH-immunoreactive cells expressing betaLH immunoreactivity in the luteal (5.9+/-0.3%) and follicular (3.4+/-0.5%) phases but no difference in the proportion of prolactin- (follicular: 2.2+/-0.7%; luteal: 2.0+/-0.8%) or TSH-immunoreactive cells (follicular: 9.6+/-3.7%; luteal: 10.8+/-2.9%) expressing betaLH. The specific function of these multihormonal gonadotropes in sheep remains to be determined.
Authors:
Mallory Mignot; Donal C Skinner
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-01-13
Journal Detail:
Title:  Cell and tissue research     Volume:  319     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-23     Completed Date:  2005-07-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  413-21     Citation Subset:  IM    
Affiliation:
Department of Zoology and Physiology, University of Wyoming, Laramie, WY, 82071-3166, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenocorticotropic Hormone / metabolism*
Animals
Estrous Cycle / metabolism
Female
Growth Hormone / metabolism*
Immunohistochemistry
Pituitary Gland, Anterior / cytology,  metabolism*
Pluripotent Stem Cells / cytology,  metabolism*
Prolactin / metabolism*
Sheep* / physiology
Thyrotropin / metabolism*
Grant Support
ID/Acronym/Agency:
5 P20 RR155553/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
9002-60-2/Adrenocorticotropic Hormone; 9002-62-4/Prolactin; 9002-71-5/Thyrotropin; 9002-72-6/Growth Hormone

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