Document Detail

Colocalization of APC and DLG at the tips of cellular protrusions in cultured epithelial cells and its dependency on cytoskeletons.
MedLine Citation:
PMID:  15609045     Owner:  NLM     Status:  MEDLINE    
Adenomatous polyposis coli gene product (APC) is a tumor suppressor linked to familial adenomatous polyposis and is thought to be involved in cellular polarization and migration in moving epithelial cells. APC interacts with the mammalian homolog of Discs large (DLG). DLG is a member of the membrane-associated guanylate kinase superfamily and is thought to function as a scaffolding protein that coordinates the assembly of a lateral plasma membrane-localized protein complex in epithelial cells. We confirmed the suitability of several anti-APC antibodies for immunocytochemical analysis. Using these antibodies, we showed that APC clusters were colocalized with DLG protein at cellular protrusions of subconfluent MDCK cells. A portion of the clusters was found at the tips of microtubules extending into the cellular protrusions. In addition, actin stress fibers converged near the clusters. When microtubules were disrupted by nocodazole, the colocalization of APC and DLG was lost due to the disappearance of APC clusters. However, the coclusters remained after depolymerization of actin filaments with latrunculin A. This is the first report showing colocalization of APC and DLG in non-polarized epithelial cells. This colocalization suggests that DLG functions not only at the lateral cell-cell contact sites of polarized epithelial cells but also at the protrusions of non-polarized epithelial cells through the interaction with APC protein.
Akiko Iizuka-Kogo; Atsushi Shimomura; Takao Senda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-12-18
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  123     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-03-03     Completed Date:  2005-07-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
Department of Anatomy I, Fujita Health University School of Medicine, Toyoake, 470-1192 Aichi, Japan.
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MeSH Terms
Adenomatous Polyposis Coli Protein / immunology,  metabolism*
Antibody Specificity
Cell Communication / physiology
Cell Line
Cell Movement
Cell Polarity
Epithelial Cells / metabolism*
Focal Adhesions / metabolism
Kidney / cytology*
Microfilaments / metabolism
Microtubules / metabolism*
Proteins / metabolism
Reg. No./Substance:
0/Adenomatous Polyposis Coli Protein; 0/Antibodies; 0/Proteins

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