Document Detail


Colloidal-gold immunocytochemical localization of osteopontin in avian eggshell gland and eggshell.
MedLine Citation:
PMID:  18256019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
During mineralization of the avian eggshell, there is a sequential and orderly deposition of both matrix and mineral phases. Therefore, the eggshell is an excellent model for studying matrix-mineral relationships and the regulation of mineralization. Osteopontin, as an inhibitor of crystal growth, potently influences the formation of calcium phosphate and calcium carbonate biominerals. The purpose of this study was to characterize matrix-mineral relationships, specifically for osteopontin, in the avian eggshell using high-resolution transmission (TEM) and scanning (SEM) electron microscopy to gain insight into how calcite crystal growth is structured and compartmentalized during eggshell mineralization. Osteopontin was localized at the ultrastructural level by colloidal-gold immunocytochemistry. In EDTA-decalcified eggshell, an extensive matrix network was observed by TEM and SEM throughout all regions and included interconnected fibrous sheets, irregularly shaped aggregates, vesicular structures, protein films, and isolated protein fibers. Osteopontin was associated with protein sheets in the highly mineralized palisades region; some of these features defined boundaries that compartmentalized different eggshell structural units. In fractured and undecalcified eggshell, osteopontin was immunolocalized on the {104} crystallographic faces of calcite-its natural cleavage plane. The specific occlusion of osteopontin into calcite during mineralization may influence eggshell structure to modify its fracture resistance.
Authors:
Maxwell T Hincke; Yung-Ching Chien; Louis C Gerstenfeld; Marc D McKee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-05
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  56     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-21     Completed Date:  2008-05-28     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  467-76     Citation Subset:  IM    
Affiliation:
Faculty of Dentistry, 3640 University Street, Montreal, Quebec, Canada H3A 2B2.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / immunology
Blotting, Western
Calcification, Physiologic
Chickens / anatomy & histology*,  immunology
Colloids
Egg Proteins / chemistry*,  immunology
Gold*
Immunohistochemistry
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Osteopontin / analysis*,  immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Colloids; 0/Egg Proteins; 0/chorion proteins; 106441-73-0/Osteopontin; 7440-57-5/Gold
Comments/Corrections

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