Document Detail


Collection and quality of rhesus monkey semen.
MedLine Citation:
PMID:  2393585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Electroejaculation is an accepted method of semen collection from nonhuman primates. Although both penile and rectal probe stimulation techniques have been used, there has been a general lack of consistency and detail regarding their application. This report describes the collection, processing, and evaluation of rhesus monkey semen contrasting two methods of penile electroejaculation: 1) a constant-voltage method where stimulus current is a variable and 2) a constant-current method where stimulus current is operator-controlled. The constant-current method was the more efficient procedure, requiring a lower stimulus current for successful electroejaculation. The influence on semen quality of potentially toxic agents used in the procedure, surgical glove powder and electrolyte cream, was tested; both were detrimental as measured by motility loss. No correlation was found between coagula volume and sperm numbers. The intra- and interanimal variability in semen samples from six monkeys was also evaluated. Penile electroejaculation, combined with control of stimulus current, provides a consistent, successful, and humane method for the collection of semen in the rhesus monkey.
Authors:
S E Lanzendorf; P M Gliessman; A E Archibong; M Alexander; D P Wolf
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  25     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1990 Jan 
Date Detail:
Created Date:  1990-10-09     Completed Date:  1990-10-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  61-6     Citation Subset:  IM    
Affiliation:
Division of Reproductive Biology and Behavior, Oregon Regional Primate Research Center, Beaverton, Oregon.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ejaculation*
Electric Stimulation*
Electrodes
Evaluation Studies as Topic
Macaca mulatta
Male
Penis / physiology
Semen / physiology*
Specimen Handling*
Sperm Motility
Grant Support
ID/Acronym/Agency:
HD23786/HD/NICHD NIH HHS; RR00163/RR/NCRR NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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