Document Detail


Collagens from the skin of arabesque greenling (Pleurogrammus azonus) solubilized with the aid of acetic acid and pepsin from albacore tuna (Thunnus alalunga) stomach.
MedLine Citation:
PMID:  20549802     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Due to the low extraction efficiency of collagen from fish skin by the typical acid solubilization process, pepsin has been widely used to aid further extraction of collagen from the residue. The aim of this study was to characterize collagen from the skin of arabesque greenling extracted with the aid of albacore tuna pepsin, in comparison with collagen obtained from the acid solubilization process. RESULTS: Acid-solubilized collagen (ASC) from the skin of arabesque greenling was extracted with acetic acid. Pepsin-solubilized collagen (PSC) was further extracted from the skin residue with the aid of pepsin from albacore tuna. The yields of ASC and PSC were 303 and 140 g kg(-1) (dry weight), respectively. Both collagens contained alpha- and beta-chains as their major components and were characterized as type I collagen. Both collagens contained glycine as a major amino acid and had imino acid content of 157-159 residues per 1000 residues. The degradation induced by lysyl endopeptidase and V8-protease was more pronounced in PSC compared with ASC. Maximal transition temperatures of both collagens were in the range of 15.4-15.7 degrees C. Fourier transform infrared spectra revealed some differences in molecular order between ASC and PSC. Nevertheless, the triple-helical structure of PSC was still predominant. Based on zeta-potential, pI of ASC and PSC was estimated to be 6.31 and 6.38, respectively. CONCLUSION: Isolation of collagens from the skin of arabesque greenling could be achieved by acid or albacore tuna pepsin solubilization. However, there was a slight difference in properties between ASC and PSC.
Authors:
Sitthipong Nalinanon; Soottawat Benjakul; Hideki Kishimura
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  1492-500     Citation Subset:  IM    
Affiliation:
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / chemistry
Amino Acids / analysis
Animals
Collagen Type I / chemistry*,  isolation & purification,  metabolism
Fish Proteins / chemistry*,  isolation & purification,  metabolism
Food Handling / methods
Industrial Waste / analysis,  economics
Isoelectric Point
Molecular Weight
Pepsin A / isolation & purification,  metabolism*
Peptide Mapping
Perciformes*
Protein Structure, Secondary
Protein Subunits / chemistry
Skin / chemistry*
Solubility
Spectroscopy, Fourier Transform Infrared
Stomach / enzymology*
Transition Temperature
Tuna*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Collagen Type I; 0/Fish Proteins; 0/Industrial Waste; 0/Protein Subunits; 64-19-7/Acetic Acid; EC 3.4.23.1/Pepsin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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