| Collagenase production at the border of granulation tissue in a healing wound: macrophage and mesenchymal collagenase production in vivo. | |
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MedLine Citation:
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PMID: 1723040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We demonstrated the cells producing collagenase and the time course of collagenase-production at early stages of wound healing, using histology and two immunohistochemical procedures on cross sections of rat skin harvested 0, 3, 5, 7 and 12 days after full-thickness incisions. A monospecific rabbit polyclonal antibody to neutral collagenase purified from rat myometrial cells was used to demonstrate collagenase production. Specificity of this reaction was confirmed by blocking the reaction with excess homogeneously purified antigen. Macrophages were simultaneously labelled using a mouse anti-rat monoclonal antibody recognizing exclusively mature macrophages. Intracellular collagenase was not reliably detectable at day 0, but was prominent at days 3 and 5 and thereafter declined. Double labeling technique showed occasional macrophages producing collagenase in the developing granulation tissue, but most cells labeled as macrophages were negative for collagenase. Most activity was found in fibroblasts adjacent to granulation tissue elements. Since the granulation tissue parallels revascularization in a dendritic pattern, a cross section at three days typically shows an annulus of collagenase-positive cells surrounding a branch of the active granulation tissue. At days 5, 7 and 12 after wounding the pattern of collagenase expression became indistinct as more tissue was involved in the granulation process. However, double-labelling for macrophages and collagenase showed the dichotomy between collagenase expression and presence of macrophages to persist. The finding that collagenase is produced in connective tissue adjacent to granulation tissue suggests an inductive process, possibly due to diffusion of cytokines produced by granulation tissue elements. |
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Authors:
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B H Porras-Reyes; H C Blair; J J Jeffrey; T A Mustoe |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Connective tissue research Volume: 27 ISSN: 0300-8207 ISO Abbreviation: Connect. Tissue Res. Publication Date: 1991 |
Date Detail:
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Created Date: 1992-03-05 Completed Date: 1992-03-05 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0365263 Medline TA: Connect Tissue Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 63-71 Citation Subset: IM |
Affiliation:
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Division of Plastic Surgery, Washington University School of Medicine, St. Louis, Missouri. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fibroblasts / enzymology Granulation Tissue / enzymology* Macrophages / metabolism* Male Microbial Collagenase / biosynthesis* Rats Skin / cytology, injuries* Staining and Labeling Wound Healing / physiology* |
| Chemical | |
Reg. No./Substance:
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EC 3.4.24.3/Microbial Collagenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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