Document Detail


Collagen tissue treated with chitosan solutions in carbonic acid for improved biological prosthetic heart valves.
MedLine Citation:
PMID:  24582232     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Calcification of bovine pericardium dramatically shortens typical lifetimes of biological prosthetic heart valves and thus precludes their choice for younger patients. The aim of the present work is to demonstrate that the calcification is to be mitigated by means of treatment of bovine pericardium in solutions of chitosan in carbonic acid, i.e. water saturated with carbon dioxide at high pressure. This acidic aqueous fluid unusually combines antimicrobial properties with absolute biocompatibility as far as at normal pressure it decomposes spontaneously and completely into H2O and CO2. Yet, at high pressures it can protonate and dissolve chitosan materials with different degrees of acetylation (in the range of 16-33%, at least) without any further pretreatment. Even exposure of the bovine pericardium in pure carbonic acid solution without chitosan already favours certain reduction in calcification, somewhat improved mechanical properties, complete biocompatibility and evident antimicrobial activity of the treated collagen tissue. The reason may be due to high extraction ability of this peculiar compressed fluidic mixture. Moreover, exposure of the bovine pericardium in solutions of chitosan in carbonic acid introduces even better mechanical properties and highly pronounced antimicrobial activity of the modified collagen tissue against adherence and biofilm formation of relevant Gram-positive and Gram-negative strains. Yet, the most important achievement is the detected dramatic reduction in calcification for such modified collagen tissues in spite of the fact that the amount of the thus introduced chitosan is rather small (typically ca. 1wt.%), which has been reliably detected using original tritium labelling method. We believe that these improved properties are achieved due to particularly deep and uniform impregnation of the collagen matrix with chitosan from its pressurised solutions in carbonic acid.
Authors:
Marat O Gallyamov; Ivan S Chaschin; Marina A Khokhlova; Timofey E Grigorev; Natalia P Bakuleva; Irina G Lyutova; Janna E Kondratenko; Gennadii A Badun; Maria G Chernysheva; Alexei R Khokhlov
Publication Detail:
Type:  Journal Article     Date:  2014-01-11
Journal Detail:
Title:  Materials science & engineering. C, Materials for biological applications     Volume:  37     ISSN:  1873-0191     ISO Abbreviation:  Mater Sci Eng C Mater Biol Appl     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-03-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101484109     Medline TA:  Mater Sci Eng C Mater Biol Appl     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  127-40     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 Elsevier B.V. All rights reserved.
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