Document Detail


Collagen in the scarless fetal skin wound: detection with picrosirius-polarization.
MedLine Citation:
PMID:  15828945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Our group has developed an ovine model of deep dermal, partial-thickness burn where the fetus heals scarlessly and the lamb heals with scar. The comparison of collagen structure between these two different mechanisms of healing may elucidate the process of scarless wound healing. Picrosirius staining followed by polarized light microscopy was used to visualize collagen fibers, with digital capture and analysis. Collagen deposition increased with fetal age and the fibers became thicker, changing from green (type III collagen) to yellow/red (type I collagen). The ratio of type III collagen to type I was high in the fetus (166), whereas the lamb had a much lower ratio (0.2). After burn, the ratios of type III to type I collagen did not differ from those in control skin for either fetus or lamb. The fetal tissue maintained normal tissue architecture after burn while the lamb tissue showed irregular collagen organization. In conclusion, the type or amount of collagen does not alter significantly after injury. Tissue architecture differed between fetal and lamb tissue, suggesting that scar development is related to collagen cross-linking or arrangement. This study indicates that healing in the scarless fetal wound is representative of the normal fetal growth pattern, rather than a "response" to burn injury.
Authors:
Leila Cuttle; Maria Nataatmadja; John F Fraser; Margit Kempf; Roy M Kimble; Mark T Hayes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society     Volume:  13     ISSN:  1067-1927     ISO Abbreviation:  Wound Repair Regen     Publication Date:    2005 Mar-Apr
Date Detail:
Created Date:  2005-04-14     Completed Date:  2005-08-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9310939     Medline TA:  Wound Repair Regen     Country:  United States    
Other Details:
Languages:  eng     Pagination:  198-204     Citation Subset:  IM    
Affiliation:
Department of Paediatrics and Child Health, University of Queensland, Royal Children's Hospital, Queensland, Australia. L.Cuttle@uq.edu.au
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MeSH Terms
Descriptor/Qualifier:
Animals
Azo Compounds / diagnostic use
Cicatrix / pathology*,  physiopathology
Collagen / physiology*
Coloring Agents
Fetus
Microscopy, Polarization / methods*
Models, Animal
Sheep
Skin / pathology*,  physiopathology
Wounds and Injuries / pathology*,  physiopathology
Chemical
Reg. No./Substance:
0/Azo Compounds; 0/Coloring Agents; 25188-41-4/C.I. direct red 80; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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