Document Detail

Collagen IV-mediated signalling is involved in progenitor Leydig cell proliferation.
MedLine Citation:
PMID:  15511338     Owner:  NLM     Status:  MEDLINE    
In rats, during postnatal Leydig cell development, the progenitor Leydig cells (PLC) proliferate actively during days 14-21 of postnatal life. Luteinizing hormone (LH) is known to stimulate Leydig cell proliferation and oestradiol 17beta inhibits this process. In order to identify the molecules involved in Leydig cell proliferation, differentially expressed genes in proliferating and non-proliferating PLC isolated from vehicle and oestradiol 17beta-treated rats respectively, were analysed by differential display reverse transcription polymerase chain reaction (DD-RT-PCR). Results revealed that the expression of collagen IV alpha4 (Col IV alpha4), a subunit of extracellular matrix (ECM) protein collagen IV, was down regulated in PLC isolated from oestradiol 17beta-treated rats. Studies on stage specific expression of Col IV alpha4 during Leydig cell development revealed that this transcript is abundantly expressed at the stage where Leydig cell proliferation is maximal and the expression of this transcript decreased during differentiation of Leydig cells, which is associated with loss of proliferation. These observations suggest that Col IV alpha4 is important for PLC proliferation. Stimulation of PLC proliferation in vitro in the presence collagen IV provides additional support for the conclusion that collagen IV-mediated signalling is involved in PLC proliferation. Further studies revealed that active forms of focal adhesion kinase (FAK) and mitogen activated protein kinase 1/2 (MAPK 1/2), the intracellular signalling molecules that are known to mediate ECM protein signalling are present only in proliferating forms of Leydig cells and are absent in non-proliferating Leydig cells. These results suggest that collagen IV-mediated signalling is involved in PLC proliferation.
M Anbalagan; A Jagannadha Rao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproductive biomedicine online     Volume:  9     ISSN:  1472-6483     ISO Abbreviation:  Reprod. Biomed. Online     Publication Date:  2004 Oct 
Date Detail:
Created Date:  2004-10-29     Completed Date:  2005-03-01     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  101122473     Medline TA:  Reprod Biomed Online     Country:  England    
Other Details:
Languages:  eng     Pagination:  391-403     Citation Subset:  IM    
Department of Biochemistry, Indian Institute of Science, Bangalore 560 012, India.
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MeSH Terms
Base Sequence
Cell Differentiation
Cell Proliferation / drug effects
Collagen Type IV / genetics,  metabolism*
DNA / genetics
Estradiol / pharmacology
Extracellular Signal-Regulated MAP Kinases / genetics
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Gene Expression Profiling
Leydig Cells / cytology*,  drug effects,  metabolism*
MAP Kinase Signaling System / drug effects
Protein-Tyrosine Kinases / genetics
RNA, Messenger / genetics,  metabolism
Rats, Wistar
Signal Transduction / drug effects
Stem Cells / cytology*,  drug effects,  metabolism*
Reg. No./Substance:
0/Collagen Type IV; 0/RNA, Messenger; 50-28-2/Estradiol; 9007-49-2/DNA; EC Adhesion Kinase 1; EC Kinases; EC Adhesion Protein-Tyrosine Kinases; EC protein, rat; EC Signal-Regulated MAP Kinases

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