Document Detail

Collaborative study for the detection of toxic compounds in shellfish extracts using cell-based assays. Part I: screening strategy and pre-validation study with lipophilic marine toxins.
MedLine Citation:
PMID:  22538779     Owner:  NLM     Status:  MEDLINE    
Human poisoning due to consumption of seafood contaminated with phycotoxins is a worldwide problem, and routine monitoring programs have been implemented in various countries to protect human consumers. Following successive episodes of unexplained shellfish toxicity since 2005 in the Arcachon Bay on the French Atlantic coast, a national research program was set up to investigate these atypical toxic events. Part of this program was devoted to fit-for-purpose cell-based assays (CBA) as complementary tools to collect toxicity data on atypical positive-mouse bioassay shellfish extracts. A collaborative study involving five laboratories was conducted. The responses of human hepatic (HepG2), human intestinal (Caco2), and mouse neuronal (Neuro2a) cell lines exposed to three known lipophilic phycotoxins-okadaic acid (OA), azaspiracid-1 (AZA1), and pectenotoxin-2 (PTX2)-were investigated. A screening strategy composed of standard operating procedures and a decision tree for dose-response modeling and assay validation were designed after a round of "trial-and-error" process. For each toxin, the shape of the concentration-response curves and the IC(50) values were determined on the three cell lines. Whereas OA induced a similar response irrespective of the cell line (complete sigmoid), PTX2 was shown to be less toxic. AZA1 induced cytotoxicity only on HepG2 and Neuro2a, but not on Caco2. Intra- and inter-laboratory coefficients of variation of cell responses were large, with mean values ranging from 35 to 54 % and from 37 to 48 %, respectively. Investigating the responses of the selected cell lines to well-known toxins is the first step supporting the use of CBA among the panel of methods for characterizing atypical shellfish toxicity. Considering these successful results, the CBA strategy will be further applied to extracts of negative, spiked, and naturally contaminated shellfish tissues.
Anne-Laure Sérandour; Aurélie Ledreux; Bénédicte Morin; Sylvain Derick; Elie Augier; Rachelle Lanceleur; Sahima Hamlaoui; Serge Moukha; Christophe Furger; Ronel Biré; Sophie Krys; Valérie Fessard; Marc Troussellier; Cécile Bernard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Validation Studies     Date:  2012-04-27
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  403     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-23     Completed Date:  2012-09-17     Revised Date:  2012-10-18    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1983-93     Citation Subset:  IM    
Unité de Toxicologie des Contaminants, ANSES, 35302 Fougères, France.
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MeSH Terms
Cell Line
Cooperative Behavior*
Dose-Response Relationship, Drug
Inhibitory Concentration 50
Marine Toxins / analysis*,  toxicity
Reg. No./Substance:
0/Marine Toxins
Comment In:
Anal Bioanal Chem. 2012 Sep;404(5):1611; author reply 1613-4   [PMID:  22797716 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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