| Collaboration of human phagocytes with LY 303366 for antifungal activity against Aspergillus fumigatus. | |
| | |
MedLine Citation:
|
PMID: 10350377 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
LY 303366, an inhibitor of 1, 3-beta-D-glucan synthase, was tested alone, or in co-culture with neutrophils or monocytes, for antifungal activity against Aspergillus fumigatus using the XTT metabolism assay. LY 303366 at 0.1 mg/L for 48 h significantly inhibited growth by conidia in a microtest plate XTT assay system. Inhibition was similar if the drug was removed after only 24 h. Microscopically this correlated with less growth and stunted malformed hyphae. LY 303366 (0.1 mg/L) also inhibited the further growth of germlings (43%) in a 24 h assay. Antifungal activity of neutrophils against 24 h control hyphal growth was limited at an effector: target ratio of 400:1. In co-cultures of neutrophils plus drug with hyphal growth from 24 h LY 303366 cultures the antifungal activity was additive. Neutrophils had a similar additive effect even if the drug were not present (i.e. when germinating conidia were pretreated with drug). Under conditions where monocytes did not have significant antifungal activity against hyphae, they collaborated with LY 303366 for significantly increased inhibition from 38% by LY 303366 alone to 67% by co-culture. Thus, LY 303366 has activity against germinating or germinated conidia of Aspergillus, human effector cells act co-operatively with LY 303366, and LY 303366 can sensitize germinating conidia for damage by host cells. |
| | |
Authors:
|
E Brummer; S D Chauhan; D A Stevens |
Related Documents
:
|
16466027 - Lichenicolins a and b, new bisnaphthopyrones from an unidentified lichenicolous fungus,... 15826047 - Detoxification of corn stover and corn starch pyrolysis liquors by ligninolytic enzymes... 16349647 - Gelatinase activity by marine fungi. 17188047 - Ocular fungal isolates and antifungal susceptibility in northern china. 2168867 - Selection of radioresistant cells by vitamin a deficiency in a small cell lung cancer c... 23250407 - Cryopreservation of somatic embryogenic cultures of pinus pinaster: effects on regrowth... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Journal of antimicrobial chemotherapy Volume: 43 ISSN: 0305-7453 ISO Abbreviation: J. Antimicrob. Chemother. Publication Date: 1999 Apr |
Date Detail:
|
Created Date: 1999-10-12 Completed Date: 1999-10-12 Revised Date: 2013-05-27 |
Medline Journal Info:
|
Nlm Unique ID: 7513617 Medline TA: J Antimicrob Chemother Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 491-6 Citation Subset: IM |
Affiliation:
|
Department of Medicine, Santa Clara Valley Medical Center, San Jose, CA 95128, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Antifungal Agents
/
pharmacology* Aspergillus fumigatus / drug effects*, growth & development, immunology Cells, Cultured Coculture Techniques Echinocandins Humans Monocytes / immunology* Neutrophils / immunology* Peptides, Cyclic / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Antifungal Agents; 0/Echinocandins; 0/Peptides, Cyclic; 9HLM53094I/anidulafungin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prediction of the antimicrobial effects of trovafloxacin and ciprofloxacin on staphylococci using an...
Next Document: Efficacy of beta-lactam and inhibitor combinations in a diffusion chamber model in rabbits.