Document Detail

Colitic scid mice fed Lactobacillus spp. show an ameliorated gut histopathology and an altered cytokine profile by local T cells.
MedLine Citation:
PMID:  16116315     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Scid mice transplanted with CD4 T blast cells develop colitis. We investigated if the disease was influenced in colitic mice treated with antibiotic and fed Lactobacillus spp. METHODS: Colitic scid mice were treated for 1 week with antibiotics (vancomycin/meropenem) followed or not followed by a 3-week administration of Lactobacillus reuteri DSM-12246 and Lactobacillus rhamnosus 19070-2 at 2x10 live bacteria/mouse/24 hours. After 12 weeks, the rectums were removed for histology, and CD4 T cells from the mesenteric lymph nodes (MLN) were polyclonally activated for cytokine measurements. RESULTS: Irrespective of no treatment or treatments with antibiotics and probiotics, all mice transplanted with T cell blasts lost 10% of their body weight during the 12-week experimental period, whereas the nontransplanted mice had a 10% weight increase (P<0.001). All mice treated with antibiotics but not fed probiotics showed severe gut inflammation, whereas only 2 of the 7 mice fed probiotics showed signs of severe colitis (P<0.05). MLN-derived CD4 T cells from this latter group of mice showed lower levels of interleukin-4 secretion (P<0.05) and a tendency to higher interferon-gamma production than mice not fed probiotics. CONCLUSIONS: Our data suggest that probiotics added to the drinking water may ameliorate local histopathological changes and influence local cytokine levels in colitic mice but not alter the colitis-associated weight loss.
Peter Lange Møller; Anders Paerregaard; Monika Gad; Nanna Ny Kristensen; Mogens Helweg Claesson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Inflammatory bowel diseases     Volume:  11     ISSN:  1078-0998     ISO Abbreviation:  Inflamm. Bowel Dis.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-23     Completed Date:  2005-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9508162     Medline TA:  Inflamm Bowel Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  814-9     Citation Subset:  IM    
Department of Dairy and Food Science, The Royal Veterinary and Agricultural University, and Department of Pediatrics, H:S Hvidovre Hospital, Copenhagen, Denmark.
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MeSH Terms
Animal Feed
Anti-Bacterial Agents
CD4-Positive T-Lymphocytes / immunology*,  transplantation
Colitis / immunology*,  therapy*
Interleukin-4 / metabolism
Lactobacillus reuteri
Mice, Inbred BALB C
Mice, SCID
Probiotics / pharmacology*
Rectum / drug effects,  immunology,  pathology*
Water Supply
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Thienamycins; 1404-90-6/Vancomycin; 207137-56-2/Interleukin-4; 96036-03-2/meropenem

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