Document Detail


Cold nights impair leaf growth and cell cycle progression in maize through transcriptional changes of cell cycle genes.
MedLine Citation:
PMID:  17208957     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Low temperature inhibits the growth of maize (Zea mays) seedlings and limits yield under field conditions. To study the mechanism of cold-induced growth retardation, we exposed maize B73 seedlings to low night temperature (25 degrees C /4 degrees C, day/night) from germination until the completion of leaf 4 expansion. This treatment resulted in a 20% reduction in final leaf size compared to control conditions (25 degrees C/18 degrees C, day/night). A kinematic analysis of leaf growth rates in control and cold-treated leaves during daytime showed that cold nights affected both cell cycle time (+65%) and cell production (-22%). In contrast, the size of mature epidermal cells was unaffected. To analyze the effect on cell cycle progression at the molecular level, we identified through a bioinformatics approach a set of 43 cell cycle genes and analyzed their expression in proliferating, expanding, and mature cells of leaves exposed to either control or cold nights. This analysis showed that: (1) the majority of cell cycle genes had a consistent proliferation-specific expression pattern; and (2) the increased cell cycle time in the basal meristem of leaves exposed to cold nights was associated with differential expression of cell cycle inhibitors and with the concomitant down-regulation of positive regulators of cell division.
Authors:
Bart Rymen; Fabio Fiorani; Fatma Kartal; Klaas Vandepoele; Dirk Inzé; Gerrit T S Beemster
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-01-05
Journal Detail:
Title:  Plant physiology     Volume:  143     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-08     Completed Date:  2007-07-25     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1429-38     Citation Subset:  IM    
Affiliation:
Department of Plant Systems Biology, Flanders Institute for Biotechnology, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Biomechanics
Cell Cycle / genetics,  physiology*
Cell Proliferation
Circadian Rhythm
Cold Temperature*
Computational Biology
Databases, Genetic
Flow Cytometry
Gene Expression Regulation, Plant*
Genes, cdc*
Plant Leaves / cytology,  genetics,  growth & development
Polymerase Chain Reaction
Zea mays / cytology,  genetics*,  growth & development
Comments/Corrections

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