| Cold adaptation in the marine bacterium, Sphingopyxis alaskensis, assessed using quantitative proteomics. | |
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MedLine Citation:
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PMID: 20482592 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cold marine environment constitutes a large proportion of the Earth's biosphere. Sphingopyxis alaskensis was isolated as a numerically abundant bacterium from several cold marine locations, and has been extensively studied as a model marine bacterium. Recently, a metabolic labelling platform was developed to comprehensively identify and quantify proteins from S. alaskensis. The approach incorporated data normalization and statistical validation for the purpose of generating highly confident quantitative proteomics data. Using this approach, we determined quantitative differences between cells grown at 10°C (low temperature) and 30°C (high temperature). Cold adaptation was linked to specific aspects of gene expression: a dedicated protein-folding system using GroESL, DnaK, DnaJ, GrpE, SecB, ClpB and PPIase; polyhydroxyalkanoate-associated storage materials; a link between enzymes in fatty acid metabolism and energy generation; de novo synthesis of polyunsaturated fatty acids in the membrane and cell wall; inorganic phosphate ion transport by a phosphate import PstB homologue; TonB-dependent receptor and bacterioferritin in iron homeostasis; histidine, tryptophan and proline amino acid metabolism; and a large number of proteins without annotated functions. This study provides a new level of understanding on how important marine bacteria can adapt to compete effectively in cold marine environments. This study is also a benchmark for comparative proteomic analyses with other important marine bacteria and other cold-adapted organisms. |
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Authors:
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Lily Ting; Timothy J Williams; Mark J Cowley; Federico M Lauro; Michael Guilhaus; Mark J Raftery; Ricardo Cavicchioli |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Environmental microbiology Volume: 12 ISSN: 1462-2920 ISO Abbreviation: Environ. Microbiol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-10-29 Completed Date: 2011-01-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883692 Medline TA: Environ Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 2658-76 Citation Subset: IM |
Copyright Information:
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© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd. |
Affiliation:
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School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, NSW 2052, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological* Amino Acids / metabolism Bacterial Proteins / metabolism Cell Membrane / physiology Cell Wall / physiology Cold Temperature* Fatty Acids / metabolism Gene Expression Regulation, Bacterial Homeostasis Iron / metabolism Protein Folding Proteome / metabolism* Seawater / chemistry, microbiology Sphingomonadaceae / isolation & purification, metabolism, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Bacterial Proteins; 0/Fatty Acids; 0/Proteome; 7439-89-6/Iron |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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