Document Detail


Coinfection of a fungal pathogen by two distinct double-stranded RNA viruses.
MedLine Citation:
PMID:  9878619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Unsegmented double-stranded (ds)RNA viruses belonging to the family Totiviridae persistently infect protozoa and fungi. In this study, two totiviruses were found to coinfect the filamentous fungus Sphaeropsis sapinea, a well known pathogen of pines. Isometric, virus-like particles approximately 35 nm in diameter were isolated from extracts of this fungus. The nucleotide sequences of the genomes of the two S. sapinea RNA viruses named SsRV1 and SsRV2 were established. The linear genomes of 5163 and 5202 bp, respectively, are identically organized with two large, overlapping ORFs. The 5' located ORF1 probably encodes the coat protein, whereas the gene product of ORF2 shows the typical features of RNA-dependent RNA polymerases. The absence of a pseudoknot and a slippery site at the overlapping region between ORF1 and ORF2, as well as the shortness of that region, leads us to suggest that the translation of ORF2 of both viruses is internally initiated. The mode of translation and the genomic organization are similar to those of Helminthosporium victoriae 190S virus (Hv190SV; Huang, S., and Ghabrial, S. A. (1996). Proc. Natl. Acad. Sci. USA 93, 12541-12546). Hv190SV thus appears to be closely related to the SsRVs. Interestingly, based on amino acid sequence homology SsRV1 is more closely related to Hv190SV than to SsRV2.
Authors:
O Preisig; B D Wingfield; M J Wingfield
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virology     Volume:  252     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-01-28     Completed Date:  1999-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  399-406     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Affiliation:
Tree Pathology Co-operative Programme, University of Pretoria, Pretoria, 0002, South Africa.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF038665;  AF039080
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Capsid / chemistry,  genetics*
Evolution, Molecular
Genome, Viral*
Mitosporic Fungi / virology*
Molecular Sequence Data
Open Reading Frames
Phylogeny*
RNA, Double-Stranded / genetics*
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Totiviridae / classification,  genetics*,  ultrastructure
Trees / microbiology
Chemical
Reg. No./Substance:
0/RNA, Double-Stranded

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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