Document Detail

Coilin levels and modifications influence artificial reporter splicing.
MedLine Citation:
PMID:  18322647     Owner:  NLM     Status:  MEDLINE    
Cajal bodies (CBs) and Gems are nuclear domains that contain factors responsible for spliceosomal small nuclear ribonucleoprotein (snRNP) biogenesis. The marker protein for CBs is coilin. In addition to snRNPs, coilin and other factors, canonical CBs contain the survivor of motor neuron protein (SMN). SMN can also localize to Gems. Considering the important role that coilin plays in the formation and composition of CBs, we tested the splicing efficiency of several cell lines that vary in regards to coilin level and modification using an artificial reporter substrate. We found that cells with both hypomethylated coilin and Gems are more efficient at reporter splicing compared to cells in which SMN localizes to CBs. In contrast, coilin reduction, which induces Gem formation, decreases cell proliferation and artificial reporter splicing. These findings demonstrate that coilin modifications or levels impact artificial reporter splicing, possibly by influencing snRNP biogenesis.
A A Whittom; H Xu; M D Hebert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  65     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-21     Completed Date:  2008-06-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1256-71     Citation Subset:  IM    
Department of Biochemistry, The University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216-4505, USA.
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MeSH Terms
Cell Proliferation
Coiled Bodies / metabolism
Gene Expression Regulation
Genes, Reporter*
Hela Cells
Mice, Knockout
Nuclear Proteins / genetics,  metabolism*
RNA Interference
RNA Precursors / metabolism
RNA Splicing / genetics*
RNA, Messenger / genetics,  metabolism
Reg. No./Substance:
0/Nuclear Proteins; 0/RNA Precursors; 0/RNA, Messenger; 136882-81-0/p80-coilin

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