Document Detail


Coil planet centrifuge - a new method for measurement of osmotic fragility of erythrocytes and its application in alcohol liver injuries.
MedLine Citation:
PMID:  6783053     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was designed to examine the osmotic resistance of erythrocytes measured by a new method-Coil Planet Centrifuge under the glutaraldehyde incubation, and to determine free cholesterol and adenine nucleotide contents of glutaraldehyde-treated erythrocytes compared with that of control erythrocytes. Glutaraldehyde increased the osmotic resistance of erythrocytes. Hemolysis end point of glutaraldehyde-treated erythrocytes shifted to hypo-osmolar side after 2.5 h hours incubation (p less than 0.001). Free cholesterol content of erythrocytes was not changed by the addition of glutaraldehyde, however, ATP content significantly increased when compared with controls (p less than 0.001). Moreover, the osmotic resistance of erythrocytes in 55 patients with alcoholic liver injuries and the relation between the disease were examined by Coil Planet Centrifuge. Hemolysis end point shifted to hypo-osmolar side in the patients, and our results suggest that the increase in osmotic resistance is partly responsible for the increase in the rigidity of erythrocytes. This new method was also helpful in evaluation in the clinical course of alcoholic liver injuries.
Authors:
H Nagata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry and experimental biology     Volume:  16     ISSN:  0366-0060     ISO Abbreviation:  Biochem Exp Biol     Publication Date:  1980  
Date Detail:
Created Date:  1981-06-23     Completed Date:  1981-06-23     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0416171     Medline TA:  Biochem Exp Biol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  3-11     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / blood
Centrifugation / methods
Cholesterol / blood
Erythrocytes*
Glutaral / pharmacology
Humans
Liver Diseases, Alcoholic / blood*
Osmotic Fragility* / drug effects
Chemical
Reg. No./Substance:
111-30-8/Glutaral; 56-65-5/Adenosine Triphosphate; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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