| Coherent movement of cell layers during wound healing by image correlation spectroscopy. | |
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MedLine Citation:
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PMID: 19804742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have determined the complex sequence of events from the point of injury until reepithelialization in axolotl skin explant model and shown that cell layers move coherently driven by cell swelling after injury. We quantified three-dimensional cell migration using correlation spectroscopy and resolved complex dynamics such as the formation of dislocation points and concerted cell motion. We quantified relative behavior such as velocities and swelling of cells as a function of cell layer during healing. We propose that increased cell volume ( approximately 37% at the basal layer) is the driving impetus for the start of cell migration after injury where the enlarged cells produce a point of dislocation that foreshadows and dictates the initial direction of the migrating cells. Globally, the cells follow a concerted vortex motion that is maintained after wound closure. Our results suggest that cell volume changes the migration of the cells after injury. |
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Authors:
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Kandice Tanner; Donald R Ferris; Luca Lanzano; Berhan Mandefro; William W Mantulin; David M Gardiner; Elizabeth L Rugg; Enrico Gratton |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 97 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-06 Completed Date: 2009-12-08 Revised Date: 2010-10-08 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 2098-106 Citation Subset: IM |
Affiliation:
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Department of Bioengineering, University of California, Berkeley, California, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Ambystoma mexicanum Animals Cell Movement* Cell Size Epidermis / injuries, pathology Humans Keratinocytes / pathology Models, Biological Spectrum Analysis Wound Healing* |
| Grant Support | |
ID/Acronym/Agency:
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5P41-RR003155/RR/NCRR NIH HHS; P41-RR01192/RR/NCRR NIH HHS; P50 GM076516-029001/GM/NIGMS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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