Document Detail


Coherent development of dermomyotome and dermis from the entire mediolateral extent of the dorsal somite.
MedLine Citation:
PMID:  12900449     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously shown that overall growth of the myotome in the mediolateral direction occurs in a coherent and uniform pattern. We asked whether development of the dermomyotome and resultant dermis follow a similar pattern or are, alternatively, controlled by restricted pools of stem cells driving directional growth. To this end, we studied cellular events that govern dermomyotome development and the regional origin of dermis. Measurements of cell proliferation, nuclear density and cellular rearrangements revealed that the developing dermomyotome can be subdivided in the transverse plane into three distinct and dynamic regions: medial, central and lateral, rather than simply into epaxial and hypaxial domains. To understand how these temporally and spatially restricted changes affect overall dermomyotome growth, lineage tracing with CM-DiI was performed. A proportional pattern of growth was measured along the entire epithelium, suggesting that mediolateral growth of the dermomyotome is coherent. Hence, they contrast with a stem cell view suggesting focal and inversely oriented sources of growth restricted to the medial and lateral edges. Consistent with this uniform mediolateral growth, lineage tracing experiments showed that the dermomyotome-derived dermis originates from progenitors that reside along the medial as well as the lateral halves of somites, and whose contribution to dermis is regionally restricted. Taken together, our results support the view that all derivatives of the dorsal somite (dermomyotome, myotome and dermis) keep a direct topographical relationship with their epithelial ascendants.
Authors:
Raz Ben-Yair; Nitza Kahane; Chaya Kalcheim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  130     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-08-05     Completed Date:  2003-11-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  4325-36     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, Hebrew University-Hadassah Medical School, Jerusalem 91120, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Patterning*
Carbocyanines / metabolism
Cell Lineage
Chick Embryo
Chimera
Dermis / cytology,  embryology*
Embryo, Nonmammalian / anatomy & histology,  physiology*
Fluorescent Dyes / metabolism
Mesoderm / physiology
Morphogenesis / physiology
Quail
Somites / cytology,  physiology*
Stem Cells / cytology,  metabolism
Chemical
Reg. No./Substance:
0/3,3'-dihexadecylindocarbocyanine; 0/Carbocyanines; 0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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