| Coherence and timing of cell cycle start examined at single-cell resolution. | |
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MedLine Citation:
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PMID: 16387649 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle "Start" in budding yeast involves induction of a large battery of G1/S-regulated genes, coordinated with bud morphogenesis. It is unknown how intra-Start coherence of these events and inter-Start timing regularity are achieved. We developed quantitative time-lapse fluorescence microscopy on a multicell-cycle timescale, for following expression of unstable GFP under control of the G1 cyclin CLN2 promoter. Swi4, a major activator of the G1/S regulon, was required for a robustly coherent Start, as swi4 cells exhibited highly variable loss of cooccurrence of regular levels of CLN2pr-GFP expression with budding. In contrast, other known Start regulators Mbp1 and Cln3 are not needed for coherence but ensure regular timing of Start onset. The interval of nuclear retention of Whi5, a Swi4 repressor, largely accounts for wild-type mother-daughter asymmetry and for variable Start timing in cln3 mbp1 cells. Thus, multiple pathways may independently suppress qualitatively different kinds of noise at Start. |
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Authors:
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James M Bean; Eric D Siggia; Frederick R Cross |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular cell Volume: 21 ISSN: 1097-2765 ISO Abbreviation: Mol. Cell Publication Date: 2006 Jan |
Date Detail:
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Created Date: 2006-01-02 Completed Date: 2006-02-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9802571 Medline TA: Mol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 3-14 Citation Subset: IM |
Affiliation:
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The Rockefeller University, New York, New York 10021, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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physiology* Cell Lineage Cyclin G Cyclins / genetics, metabolism DNA-Binding Proteins Gene Expression Regulation, Fungal Microscopy, Fluorescence / methods Recombinant Fusion Proteins / genetics, metabolism Repressor Proteins / genetics, metabolism Saccharomyces cerevisiae / cytology*, genetics, physiology* Saccharomyces cerevisiae Proteins / genetics, metabolism Time Factors Transcription Factors / genetics, metabolism Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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GM047238/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CLN2 protein, S cerevisiae; 0/Cyclin G; 0/Cyclins; 0/DNA-Binding Proteins; 0/Recombinant Fusion Proteins; 0/Repressor Proteins; 0/SWI4 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/Whi5 protein, S cerevisiae |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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