| Cognate interactions between helper T cells and B cells. III. Contact-dependent, lymphokine-independent induction of B cell cycle entry by activated helper T cells. | |
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MedLine Citation:
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PMID: 2476483 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An Ag-specific, IL-2-dependent Th clone induced the growth of B cells in a class II-restricted, Ag-specific, IL-2-dependent manner. The formation of stable Th-3.1-B cell conjugates was restricted by Ag and class II MHC. After activation of Th-3.1 by insolubilized anti-T3 (Th-3.1T3), Th-3.1T3 induced the growth of B cells in a class II unrestricted, Ag nonspecific manner. The formation of stable conjugates between Th-3.1T3 and B cells was also class II unrestricted and Ag nonspecific. Although the interaction of Th-3.1T3 and B cells was class II unrestricted, the interaction was inhibited by the combination of anti-IA and anti-IE mAb. This suggested that monomorphic domains of class II MHC molecules were involved in Th-3.1T3-B cell interaction. Fixed Th-3.1T3 but not fixed resting Th-3.1 induced B cell cycle entry, as measured by an increase in B cell RNA synthesis. Trypsin-treatment of Th-3.1T3 before fixation reduced their ability to activate B cells, indicating that cell surface proteins on Th-3.1T3 were required for enhanced B cell RNA synthesis. Anti-IL-4, anti-IL-2R, or anti-IFN-gamma did not affect the ability of Th-3.1T3 to induce heightened B cell RNA synthesis. Progression into S phase by B cells activated with fixed Th-3.1T3 was supported by the addition of soluble factors. When stimulated with fixed Th-3.1T3, EL4 supernatant (SN) enhanced B cell DNA synthesis. Depletion of IL-4, but not IL-2, from EL4 SN ablated its supportive capabilities. IL-4 alone was completely ineffective in supporting entry into S phase. Therefore, IL-4 and another activity(ies) in EL4 SN were necessary for B cell cycle progression into S phase. Taken together, these data suggest that after Th activation, Th cell surface proteins are expressed that mediate the binding of Th to B cells via recognition of nonpolymorphic domains of class II MHC molecules. Contact of Th-3.1T3 with B cells, not lymphokines, results in the entry of B cells into the cell cycle and heightened B cell lymphokine responsiveness. The addition of exogenous lymphokines supports the progression of Th-3.1T3-activated B cells into S phase. |
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Authors:
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R J Noelle; J McCann; L Marshall; W C Bartlett |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 143 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1989 Sep |
Date Detail:
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Created Date: 1989-10-18 Completed Date: 1989-10-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1807-14 Citation Subset: AIM; IM |
Affiliation:
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Department of Microbiology, Dartmouth Medical School, Hanover, NH 03755. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / physiology Antigens, CD3 Antigens, Differentiation, T-Lymphocyte / immunology B-Lymphocytes / immunology*, physiology Cell Communication* Cell Cycle* DNA / biosynthesis Epitopes Female Fixatives Histocompatibility Antigens Class II Interferon-gamma / physiology Interleukin-4 Interleukins / physiology Interphase Lymphocyte Activation* Lymphokines / physiology* Mice Mice, Inbred C57BL Mice, Inbred DBA Receptors, Antigen, T-Cell / immunology Receptors, Interleukin-2 / physiology T-Lymphocytes, Helper-Inducer / immunology*, physiology |
| Grant Support | |
ID/Acronym/Agency:
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GM-36814/GM/NIGMS NIH HHS; GM37767/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD3; 0/Antigens, Differentiation, T-Lymphocyte; 0/Epitopes; 0/Fixatives; 0/Histocompatibility Antigens Class II; 0/Interleukins; 0/Lymphokines; 0/Receptors, Antigen, T-Cell; 0/Receptors, Interleukin-2; 207137-56-2/Interleukin-4; 82115-62-6/Interferon-gamma; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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