| Coffee consumption enhances high-density lipoprotein-mediated cholesterol efflux in macrophages. | |
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MedLine Citation:
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PMID: 20075335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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RATIONALE: Association of habitual coffee consumption with coronary heart disease morbidity and mortality has not been established. We hypothesized that coffee may enhance reverse cholesterol transport (RCT) as the antiatherogenic properties of high-density lipoprotein (HDL). OBJECTIVE: This study was to investigate whether the phenolic acids of coffee and coffee regulates RCT from macrophages in vitro, ex vivo and in vivo. METHODS AND RESULTS: Caffeic acid and ferulic acid, the major phenolic acids of coffee, enhanced cholesterol efflux from THP-1 macrophages mediated by HDL, but not apoA-I. Furthermore, these phenolic acids increased both the mRNA and protein levels of ATP-binding cassette transporter (ABC)G1 and scavenger receptor class B type I (SR-BI), but not ABCA1. Eight healthy volunteers were recruited for the ex vivo study, and blood samples were taken before and 30 minutes after consumption of coffee or water in a crossover study. The mRNA as well as protein levels of ABCG1, SR-BI, and cholesterol efflux by HDL were increased in the macrophages differentiated under autologous sera obtained after coffee consumption compared to baseline sera. Finally, effects of coffee and phenolic acid on in vivo RCT were assessed by intraperitoneally injecting [(3)H]cholesterol-labeled acetyl low-density lipoprotein-loaded RAW264.7 cells into mice, then monitoring appearance of (3)H tracer in plasma, liver, and feces. Supporting in vitro and ex vivo data, ferulic acid was found to significantly increase the levels of (3)H tracer in feces. CONCLUSIONS: Coffee intake might have an antiatherogenic property by increasing ABCG1 and SR-BI expression and enhancing HDL-mediated cholesterol efflux from the macrophages via its plasma phenolic acids. |
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Authors:
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Harumi Uto-Kondo; Makoto Ayaori; Masatsune Ogura; Kazuhiro Nakaya; Mai Ito; Atsushi Suzuki; Shun-ichi Takiguchi; Emi Yakushiji; Yoshio Terao; Hideki Ozasa; Tetsuya Hisada; Makoto Sasaki; Fumitaka Ohsuzu; Katsunori Ikewaki |
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Publication Detail:
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Type: Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-14 |
Journal Detail:
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Title: Circulation research Volume: 106 ISSN: 1524-4571 ISO Abbreviation: Circ. Res. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-03-05 Completed Date: 2010-03-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: United States |
Other Details:
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Languages: eng Pagination: 779-87 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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ATP-Binding Cassette Transporters
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genetics,
metabolism Adult Animals Apolipoprotein A-I / metabolism Beverages* Bile / metabolism Biological Transport Caffeic Acids / blood, pharmacology* Cell Line Cholesterol / blood, metabolism* Coffee* Coronary Disease / metabolism, prevention & control Coumaric Acids / blood, pharmacology* Cross-Over Studies Dose-Response Relationship, Drug Feces / chemistry Female Genes, Reporter Humans Lipoproteins, HDL / blood, metabolism* Lipoproteins, LDL / metabolism Liver / metabolism Macrophages / drug effects*, metabolism Male Mice Mice, Inbred C57BL Middle Aged RNA, Messenger / metabolism Scavenger Receptors, Class B / genetics, metabolism Time Factors Transfection Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/ABCG1 protein, human; 0/APOA1 protein, human; 0/ATP binding cassette transporter 1; 0/ATP-Binding Cassette Transporters; 0/Apolipoprotein A-I; 0/Caffeic Acids; 0/Coffee; 0/Coumaric Acids; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL; 0/RNA, Messenger; 0/SCARB1 protein, human; 0/Scavenger Receptors, Class B; 0/acetyl-LDL; 1135-24-6/ferulic acid; 331-39-5/caffeic acid; 57-88-5/Cholesterol |
| Comments/Corrections | |
Comment In:
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Circ Res. 2010 Mar 5;106(4):627-9
[PMID:
20203311
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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