Document Detail

Coexpression of multiple genes reconstitutes two pathways of very long-chain polyunsaturated fatty acid biosynthesis in Pichia pastoris.
MedLine Citation:
PMID:  24863294     Owner:  NLM     Status:  Publisher    
The introduction of novel traits to cells often requires the stable coexpression of multiple genes within the same cell. Herein, we report that C22 very long-chain polyunsaturated fatty acids (VLC-PUFAs) were synthesized from C18 precursors by reactions catalyzed by delta 6-desaturase, an ELOVL5 involved in VLC-PUFA elongation, and delta 5-desaturase. The coexpression of McD6DES, AsELOVL5, and PtD5DES encoding the corresponding enzymes, produced docosatetraenoic acid (C22:4 n-6) and docosapentaenoic acid (C22:5 n-3), as well as arachidonic acid (C20:4 n-6) and eicosapentaenoic acid (C20:5 n-3) in the methylotrophic yeast Pichia pastoris. The expression of each gene increased within 24 h, with high transcript levels after induction with 0.5 or 1 % methanol. High levels of the newly expressed VLC-PUFAs occurred after 144 h. This expression system exemplifies the recent progress and future possibilities of the metabolic engineering of VLC-PUFAs in oilseed crops.
Sun Hee Kim; Kyung Hee Roh; Kwang-Soo Kim; Hyun Uk Kim; Kyeong-Ryeol Lee; Han-Chul Kang; Jong-Bum Kim
Related Documents :
10552474 - Assessment of the antioxidant potential of scotch whiskeys by electron spin resonance s...
25279314 - Cardiac taurine and principal amino acids in right and left ventricles of patients with...
21905284 - Anti-inflammatory effects of scoparia dulcis l. and betulinic acid.
22274694 - Modulation of the domain topography of biphasic monolayers of stearic acid and dimyrist...
24235104 - Changes in breath sound power spectra during experimental oleic acid-induced lung injur...
17519284 - Metabolic challenges reveal impaired fatty acid metabolism and translocation of fat/cd3...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-5-27
Journal Detail:
Title:  Biotechnology letters     Volume:  -     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2014 May 
Date Detail:
Created Date:  2014-5-27     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Identification of soybean MYC2-like transcription factors and overexpression of GmMYC1 could stimula...
Next Document:  Chimeric promoter mediates guard cell-specific gene expression in tobacco under water deficit.