| Coevolution of cells and viruses in a persistent infection of foot-and-mouth disease virus in cell culture. | |
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MedLine Citation:
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PMID: 2835509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Virus and cells evolve during serial passage of cloned BHK-21 cells persistently infected with foot-and-mouth disease virus (FMDV). These carrier cells, termed C1-BHK-Rc1 (J.C. de la Torre, M. Dávila, F. Sobrino, J. Ortín, and E. Domingo, Virology 145:24-35, 1985), become constitutively resistant to the parental FMDV C-S8c1. Curing of late-passage C1-BHK-Rc1 cells of FMDV by ribavirin treatment (J.C. de la Torre, B. Alarcón, E. Martínez-Salas, L. Carrasco, and E. Domingo, J. Virol. 61:233-235, 1987) did not restore sensitivity to FMDV C-S8c1. The resistance of C1-BHK-Rc1 cells to FMDV C-S8c1 was not due to an impairment of attachment, penetration, or uncoating of the particles but to some intracellular block that resulted in a 100-fold decrease in the amount of FMDV RNA in the infected cells. FMDV R59, the virus isolated from late-passage carrier cells, partly overcame the cellular block and was more cytolytic than FMDV C-S8c1 for BHK-21 cells. Sequencing of the VP1 gene from nine viral clones from C1-BHK-Rc1 cells showed genetic heterogeneity of 5 X 10(-4) substitutions per nucleotide. Mutations were sequentially fixed during persistence. In addition to resistance to FMDV C-S8c1, C1-BHK-Rc1 cells showed a characteristic round cell morphology, and compared with BHK-21 cells, they grew faster in liquid culture, were less subject to contact inhibition of growth, and had an increased ability to form colonies in semisolid agar. Reconstitution of a persistent infection was readily attained with late-passage C1-BHK-Rc1 cells and FMDV C-S8c1 or FMDV R59. The results suggest that coevolution of BHK-21 cells and FMDV contributes to the maintenance of persistence in cell culture. |
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Authors:
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J C de la Torre; E Martínez-Salas; J Diez; A Villaverde; F Gebauer; E Rocha; M Dávila; E Domingo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of virology Volume: 62 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1988 Jun |
Date Detail:
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Created Date: 1988-06-20 Completed Date: 1988-06-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2050-8 Citation Subset: IM |
Affiliation:
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Centro de Biología Molecular (Consejo Superior de Investigaciones Científicas), Universidad Autónoma de Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aphthovirus / genetics*, growth & development Base Sequence Cell Division Cell Line Cells, Cultured / cytology, microbiology Cricetinae Endocytosis Molecular Sequence Data Mutation RNA, Viral / biosynthesis Time Factors Transfection Virus Replication |
| Chemical | |
Reg. No./Substance:
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0/RNA, Viral |
| Comments/Corrections | |
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