Document Detail


Coenzyme function of cobalamin analogues from calf kidney.
MedLine Citation:
PMID:  6375765     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Calf kidney has been used as a tissue source for the isolation of cobalamin analogues, which are defined here as cobalt-containing compounds of distinctive chromatographic behavior that are extractable from tissues by methods conventionally used to extract cobalamin and which, in radioisotope dilution assays, are more active with R-protein as binder than intrinsic factor and are relatively less active in microbiologic assays. Preparatory methods employed reverse affinity chromatography or a series of chemical extractions for the isolation of corrin followed by Dowex-50 chromatography. An analogue-containing fraction (peak 2) was eluted by acetate buffers between pH 4 and 5. This material was shown to contain cobalt, to migrate differently than the four cobalamins in Dowex-50 and paper chromatography, and to display a pattern of properties that is compatible with the above definition of cobalamin analogues. These analogues stimulated crude preparations of N5-methyltetrahydrofolate-homocysteine methyltransferase (EC 2.1.1.13) from Escherichia coli and rat liver at far lower concentrations (1-40 nmol/L) than the major cobalamins. No evidence of enzymatic conversion of cobalamin to analogue could be demonstrated.
Authors:
J Thorndike; W S Beck
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood     Volume:  64     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1984 Jul 
Date Detail:
Created Date:  1984-07-30     Completed Date:  1984-07-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  91-8     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase / metabolism
Animals
Cattle
Chromatography
Cobalt / analysis
Coenzymes / metabolism*
Corrinoids
Escherichia coli / enzymology
Kidney / enzymology*
Vitamin B 12 / analogs & derivatives*,  analysis,  isolation & purification
Grant Support
ID/Acronym/Agency:
AM-26775/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Coenzymes; 0/Corrinoids; 68-19-9/Vitamin B 12; 7440-48-4/Cobalt; EC 2.1.1.13/5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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