Document Detail


Coenzyme Q addition to an n-6 PUFA-rich diet resembles benefits on age-related mitochondrial DNA deletion and oxidative stress of a MUFA-rich diet in rat heart.
MedLine Citation:
PMID:  19948181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Age-related changes in cardiomyocytes reduce the capacity to recover from acute injury or to adapt during chronic disease in advanced age. N-6 polyunsaturated fatty acids (n-6PUFA) lead to higher lipid peroxidation during aging than the less oxidizable monounsaturated fatty acids (MUFA); and coenzyme Q (CoQ)-supplemented n-6PUFA lengthens the lifespan and reduces peroxidation in comparison to non-supplemented n-6PUFA. Here, lifelong feeding on MUFA, n-6PUFA, and n-6 PUFA+CoQ was compared regarding age-related alterations in rat heart. Less mitochondrial area and perimeter were reported for aged n-6 PUFA-fed animals while MUFA led to a higher density of mitochondrial cristae. Mitochondrial complexes and cytochrome c oxidase activity decreased with aging (except complex I and cytochrome c oxidase in n-6 PUFA+CoQ), while increased apoptosis-inducing factor was found with aging. MUFA led to lower mitochondrial DNA-deletion frequency. The lowest hydroperoxide levels for aged animals were found for n-6 PUFA+CoQ, which also showed lower concentrations than did n-6 PUFA. For protein oxidation, specific carbonyl compounds were lower in aged animals; meanwhile lipoxidation-derived protein-oxidation markers were higher. The results suggest that MUFA can protect mitochondria from age-related changes, and that CoQ supplementation to n-6 PUFA partially resembles MUFA benefits. Moreover, under our experimental conditions, lipid-derived oxidative damage appears to be more important than the pure protein-derived oxidative damage during aging.
Authors:
Jos? L Quiles; Reinald Pamplona; M Carmen Ramirez-Tortosa; Alba Naud?; Manuel Portero-Otin; Eduardo Araujo-Nepomuceno; Magdalena L?pez-Fr?as; Maurizio Battino; Julio J Ochoa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-03
Journal Detail:
Title:  Mechanisms of ageing and development     Volume:  131     ISSN:  1872-6216     ISO Abbreviation:  Mech. Ageing Dev.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-04-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0347227     Medline TA:  Mech Ageing Dev     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  38-47     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Institute of Nutrition and Food Technology Jos? Mataix Verd?, Department of Physiology, University of Granada, Granada, Spain. jlquiles@ugr.es
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MeSH Terms
Descriptor/Qualifier:
Age Factors
Aging / genetics,  metabolism
Animals
Apoptosis Inducing Factor / metabolism
DNA, Mitochondrial / metabolism*
Diet*
Dietary Supplements*
Electron Transport Chain Complex Proteins / metabolism
Fatty Acids, Monounsaturated / administration & dosage,  metabolism*
Fatty Acids, Omega-6 / administration & dosage,  metabolism*
Gene Deletion
Lipid Peroxidation / drug effects
Male
Mitochondria, Heart / drug effects*,  metabolism,  ultrastructure
Myocytes, Cardiac / drug effects*,  metabolism,  ultrastructure
Oxidative Stress / drug effects*
Plant Oils / metabolism
Protein Carbonylation / drug effects
Rats
Rats, Wistar
Ubiquinone / administration & dosage*
Chemical
Reg. No./Substance:
0/Apoptosis Inducing Factor; 0/DNA, Mitochondrial; 0/Electron Transport Chain Complex Proteins; 0/Fatty Acids, Monounsaturated; 0/Fatty Acids, Omega-6; 0/Pdcd8 protein, rat; 0/Plant Oils; 1339-63-5/Ubiquinone; 8001-21-6/sunflower seed oil; 8001-25-0/olive oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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