Document Detail

Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modification.
MedLine Citation:
PMID:  3054566     Owner:  NLM     Status:  MEDLINE    
An Escherichia coli isoleucine transfer RNA specific for the codon AUA (tRNA(2Ile) or tRNA(minorIle] has a novel modified nucleoside, lysidine in the first position of the anticodon (position 34), which is essential for the specific recognition of the codon AUA. We isolated the gene for tRNA(2Ile) (ileX) and found that the anticodon is CAT, which is characteristic of the methionine tRNA gene. Replacement of L(34) of tRNA(2Ile) molecule enzymatically with unmodified C(34) resulted in a marked reduction of the isoleucine-accepting activity and, surprisingly, in the appearance of methionine-accepting activity. Thus, both the codon and amino-acid specificity of this tRNA are converted by a single post-transcriptional modification of the first position of the anticodon during tRNA maturation.
T Muramatsu; K Nishikawa; F Nemoto; Y Kuchino; S Nishimura; T Miyazawa; S Yokoyama
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature     Volume:  336     ISSN:  0028-0836     ISO Abbreviation:  Nature     Publication Date:  1988 Nov 
Date Detail:
Created Date:  1988-12-19     Completed Date:  1988-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410462     Medline TA:  Nature     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  179-81     Citation Subset:  IM    
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
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MeSH Terms
Base Sequence
Escherichia coli / genetics*
Molecular Sequence Data
RNA Processing, Post-Transcriptional*
RNA, Messenger*
RNA, Transfer, Amino Acid-Specific / genetics*
RNA, Transfer, Ile / genetics
Reg. No./Substance:
0/Anticodon; 0/Codon; 0/RNA, Messenger; 0/RNA, Transfer, Amino Acid-Specific; 0/RNA, Transfer, Ile

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