| Coculturing posterior pituitary and GH3 cells: dramatic stimulation of prolactin gene expression. | |
| | |
MedLine Citation:
|
PMID: 8419149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recent evidence suggests that the posterior pituitary (PP), also called the neurointermediate lobe, regulates PRL release. We previously reported that cocultures of anterior pituitary and PP cells resulted in a 2- to 3-fold increase in PRL content and release. For this study we chose GH3 cells (a somatomammotroph tumor cell line) to determine whether coculturing GH3 with PP cells: 1) stimulates the release and cell content of PRL as compared with GH; 2) increases GH3 cell proliferation; and 3) affects PRL messenger RNA (mRNA) levels. Exp 1. GH3 cells (25,000 cells per well; 25K) were cocultured with PP cells (0K, 12.5K, or 25K) from male rats in serum-free media for 1, 2, 4, and 7 days; hormones were measured by RIA. Coculturing resulted in 5- to 10-fold increases in both media and cell PRL that were linear with time and dependent on the number of PP cells. In contrast, media GH increased only 1.5- to 2-fold, and GH cell content reduced by 75%. Exp 2. GH3, PP, and GH3 + PP cells were cultured for 1, 2, and 4 days and then incubated with [3H]thymidine for 5 h. The incorporation of [3H]thymidine in GH3 cells remained constant over time and showed a small, early increase in cocultures. In contrast, incubation of PP cells alone resulted in a 50- to 60-fold rise in [3H]thymidine incorporation from days 1-4 in culture. Exp 3. Cytoplasmic mRNA was determined by slot blot hybridization with 32P-labeled complementary DNA probes for PRL and GH. After coculturing 25K GH3 cells with 12.5K and 25K PP cells for 4 days, PRL mRNA levels increased 15- and 30-fold, respectively, whereas GH mRNA levels rose less than 2-fold. Neither PRL nor GH mRNA were detected in PP cells. Conclusions: 1) coculturing GH3 with PP cells dramatically stimulates PRL gene expression, synthesis, and release; 2) this response is specific for PRL, has little effect on GH, and is not due to increased GH3 cell proliferation; and 3) we speculate that a subpopulation of intermediate lobe cells, possibly with a proliferative capacity, is responsible for inducing these effects. |
| | |
Authors:
|
A Corcia; R Steinmetz; J W Liu; N Ben-Jonathan |
Related Documents
:
|
12849969 - Ontogeny of adenohypophyseal cells in the pituitary of the american shad (alosa sapidis... 10873299 - Steroid hormone (hydrocortisone, oestradiol and testosterone) uptake, storage or induce... 367849 - Cell membrane, a target for steroid hormones. 2186959 - Salmonid pituitary gonadotrophs. ii. ontogeny of gth i and gth ii cells in the rainbow ... 4050909 - Hormonal and morphologic effects of bromocriptine on normal rat pituitary and gh3 tumor... 9224529 - Immunocytochemical study of pituitary oncocytic adenomas. 19003139 - In vitro neuroprotective action of recombinant rat erythropoietin produced by astrocyte... 11058449 - Ultrastructure of human blastocyst-endometrial interactions in vitro. 7691179 - Optical imaging of cl- permeabilities in normal and cftr-expressing mouse l cells. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Endocrinology Volume: 132 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1993 Jan |
Date Detail:
|
Created Date: 1993-02-05 Completed Date: 1993-02-05 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 80-5 Citation Subset: AIM; IM |
Affiliation:
|
Department of Physiology and Biophysics, Indiana University School of Medicine, Indianapolis 46202. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blotting, Northern Cells, Cultured DNA / biosynthesis Gene Expression* Growth Hormone / genetics, metabolism Male Nucleic Acid Hybridization Pituitary Gland, Posterior / physiology* Prolactin / genetics*, metabolism RNA, Messenger / analysis, metabolism Rats Rats, Wistar Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
|
DK-39551/DK/NIDDK NIH HHS; NS-13243/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 9002-62-4/Prolactin; 9002-72-6/Growth Hormone; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Alteration of phosphotyrosine phosphatase activity in tissues from diabetic and pregnant rats.
Next Document: Structural basis of the mutagenicity of heterocyclic amines formed during the cooking processes.