Document Detail


Coculturing embryonic stem cells with damaged hepatocytes leads to restoration of damage and high frequency of fusion.
MedLine Citation:
PMID:  20003813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Controversy surrounds issue of cell fusion as a repair mechanism whereby stem cells regenerate. To identify the ratio of fusion happens between stem cells and damaged cells, hepatic cells were damaged with 200microM H2O2 for 2 hr. Then, mouse ESCs were cocultured with damaged human hepatocytes. Fusion was detected directly by karyotyping after 48hr coculture as well as by Oct4 promoter drove GFP signal. Results showed that average ratio of fusion in undamaged control group was 0.031 per thousand while ratio of fusion in damaged group was 0.357 per thousand, which was 10 times higher than fusion happened in the control group. Meanwhile, GFP signal indicated that fusion induced hepatic cells' Oct-4 reactivation. Fusion derived hybrid cells contained chromosomes from both parental cells. Most of the chromosomes were from damaged human hepatic cells. Activity of damage-related enzymes LDH, SGOT and SGPT were significantly lower at 48hr coculture than at 12hr coculture. Expression of albumin in co-culture system was up-regulated after coculture, which indicated the reparation of damage after coculturing. Also, by applying RT-PCR and immunocytochemistry differentiation status of ES cells were evaluated. It was shown that ES cells differentiated to hepatic lineage cells and expressed hepatic genes and proteins.
Authors:
D Xu; F Wang; Z Pan; Q Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-15
Journal Detail:
Title:  Cellular and molecular biology (Noisy-le-Grand, France)     Volume:  55 Suppl     ISSN:  1165-158X     ISO Abbreviation:  Cell. Mol. Biol. (Noisy-le-grand)     Publication Date:  2009  
Date Detail:
Created Date:  2009-12-16     Completed Date:  2010-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9216789     Medline TA:  Cell Mol Biol (Noisy-le-grand)     Country:  France    
Other Details:
Languages:  eng     Pagination:  OL1186-99     Citation Subset:  IM    
Affiliation:
Center of Embryo Engineering and Technology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Death / drug effects
Cell Fusion
Cells, Cultured
Coculture Techniques
Embryonic Stem Cells / cytology*,  metabolism
Hepatocytes / cytology*,  drug effects,  metabolism
Humans
Hydrogen Peroxide / pharmacology
Mice
Chemical
Reg. No./Substance:
7722-84-1/Hydrogen Peroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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