Document Detail


Cobalt chloride induces apoptosis and zinc chloride suppresses cobalt-induced apoptosis by Bcl-2 expression in human submandibular gland HSG cells.
MedLine Citation:
PMID:  17786326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To determine the effects of cobalt chloride on human submandibular gland cells, HSG cells were exposed to various concentrations of cobalt chloride. Cobalt chloride induced cytotoxicity and cell death in HSG cells as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, marked nuclear condensation and fragmentation of chromatin were observed in cobalt chloride-treated cells. Cobalt chloride induced DNA ladder formation in HSG cells in both dose- and time-dependent manner with maximal effect at a concentration of 0.5 mM and 48 h, respectively. Cobalt chloride inhibited the expression of both Bcl-2 protein and mRNA in dose- and time-dependent manner. Zinc chloride recovered the cobalt-suppressed Bcl-2 expression and protected against cobalt-induced apoptosis in HSG cells. Our results show that the pathway of the apoptosis in HSG cells is regulated by cobalt chloride and zinc chloride. Our results also indicate that cobalt-induced apoptotic steps in HSG cells are related to the production of Bcl-2 protein.
Authors:
Kazumi Akita; Hirohiko Okamura; Kaya Yoshida; Hiroyuki Morimoto; Hiroaki Ogawa-Iyehara; Tatsuji Haneji
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  31     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-05     Completed Date:  2007-11-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  923-9     Citation Subset:  IM    
Affiliation:
Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8504, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antimutagenic Agents / pharmacology*
Apoptosis / drug effects*
Blotting, Western
Cell Nucleus / metabolism
Cell Survival / drug effects
Cells, Cultured / drug effects
Chlorides / pharmacology*
Cobalt / pharmacology*
Humans
Mouthwashes / pharmacology*
Proto-Oncogene Proteins c-bcl-2 / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Salivary Ducts / cytology
Submandibular Gland / cytology,  drug effects*
Zinc Compounds / pharmacology*
Chemical
Reg. No./Substance:
0/Antimutagenic Agents; 0/Chlorides; 0/Mouthwashes; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/Zinc Compounds; 7440-48-4/Cobalt; 7646-79-9/cobaltous chloride; 7646-85-7/zinc chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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