| Cobalt chloride induces apoptosis and zinc chloride suppresses cobalt-induced apoptosis by Bcl-2 expression in human submandibular gland HSG cells. | |
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MedLine Citation:
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PMID: 17786326 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the effects of cobalt chloride on human submandibular gland cells, HSG cells were exposed to various concentrations of cobalt chloride. Cobalt chloride induced cytotoxicity and cell death in HSG cells as determined by phase-contrast microscopy and WST-1 cell viability assay. By using the Hoechst 33342 staining, marked nuclear condensation and fragmentation of chromatin were observed in cobalt chloride-treated cells. Cobalt chloride induced DNA ladder formation in HSG cells in both dose- and time-dependent manner with maximal effect at a concentration of 0.5 mM and 48 h, respectively. Cobalt chloride inhibited the expression of both Bcl-2 protein and mRNA in dose- and time-dependent manner. Zinc chloride recovered the cobalt-suppressed Bcl-2 expression and protected against cobalt-induced apoptosis in HSG cells. Our results show that the pathway of the apoptosis in HSG cells is regulated by cobalt chloride and zinc chloride. Our results also indicate that cobalt-induced apoptotic steps in HSG cells are related to the production of Bcl-2 protein. |
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Authors:
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Kazumi Akita; Hirohiko Okamura; Kaya Yoshida; Hiroyuki Morimoto; Hiroaki Ogawa-Iyehara; Tatsuji Haneji |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 31 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2007 Oct |
Date Detail:
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Created Date: 2007-09-05 Completed Date: 2007-11-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 923-9 Citation Subset: IM |
Affiliation:
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Department of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8504, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antimutagenic Agents
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pharmacology* Apoptosis / drug effects* Blotting, Western Cell Nucleus / metabolism Cell Survival / drug effects Cells, Cultured / drug effects Chlorides / pharmacology* Cobalt / pharmacology* Humans Mouthwashes / pharmacology* Proto-Oncogene Proteins c-bcl-2 / genetics, metabolism* RNA, Messenger / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Salivary Ducts / cytology Submandibular Gland / cytology, drug effects* Zinc Compounds / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antimutagenic Agents; 0/Chlorides; 0/Mouthwashes; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/Zinc Compounds; 7440-48-4/Cobalt; 7646-79-9/cobaltous chloride; 7646-85-7/zinc chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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