| Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(epsilon-caprolactone) nanofibers for sustained release. | |
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MedLine Citation:
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PMID: 16602720 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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As an aim toward developing biologically mimetic and functional nanofiber-based tissue engineering scaffolds, we demonstrated the encapsulation of a model protein, fluorescein isothiocyanate-conjugated bovine serum albumin (fitcBSA), along with a water-soluble polymer, poly(ethylene glycol) (PEG), within the biodegradable poly(epsilon-caprolactone) (PCL) nanofibers using a coaxial electrospinning technique. By variation of the inner flow rates from 0.2 to 0.6 mL/h with a constant outer flow rate of 1.8 mL/h, fitcBSA loadings of 0.85-2.17 mg/g of nanofibrous membranes were prepared. Variation of flow rates also resulted in increases of fiber sizes from ca. 270 nm to 380 nm. The encapsulation of fitcBSA/PEG within PCL was subsequently characterized by laser confocal scanning microscopy, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) analysis. In vitro release studies were conducted to evaluate sustained release potential of the core-sheath-structured composite nanofiber PCL-r-fitcBSA/PEG. As a negative control, composite nanofiber PCL/fitcBSA/PEG blend was prepared from a normal electrospinning method. It was found that core-sheath nanofibers PCL-r-fitcBSA/PEG pronouncedly alleviated the initial burst release for higher protein loading and gave better sustainability compared to that of PCL/fitcBSA/PEG nanofibers. The present study would provide a basis for further design and optimization of processing conditions to control the nanostructure of core-sheath composite nanofibers and ultimately achieve desired release kinetics of bioactive proteins (e.g., growth factors) for practical tissue engineering applications. |
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Authors:
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Y Z Zhang; X Wang; Y Feng; J Li; C T Lim; S Ramakrishna |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biomacromolecules Volume: 7 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-04-10 Completed Date: 2006-07-03 Revised Date: 2008-08-14 |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 1049-57 Citation Subset: IM |
Affiliation:
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Division of Bioengineering, Department of Mechanical Engineering, and Nanoscience and Nanotechnology Initiative, National University of Singapore, 9 Engineering Drive 1, Singapore 117576. biezyz@nus.edu.sg |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Electrochemistry Fluorescein-5-isothiocyanate / chemistry* Nanostructures / chemistry*, ultrastructure Nanotechnology / instrumentation, methods* Particle Size Polyesters / chemistry* Serum Albumin, Bovine / chemistry* Surface Properties Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Polyesters; 0/Serum Albumin, Bovine; 24980-41-4/polycaprolactone; 3326-32-7/Fluorescein-5-isothiocyanate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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