Document Detail


Polydopamine/Liposome Coatings and Their Interaction with Myoblast Cells.
MedLine Citation:
PMID:  21539399     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Surface-mediated drug delivery is a recent concept, where active surface coatings are employed to deliver therapeutic cargo to cells. Herein, we explore the potential of liposomes embedded in polydopamine (PDA) coatings to serve as drug deposits stored on planar substrates. We quantify the PDA growth rate on glass by XPS and show that PDA coatings support myoblast adherence and proliferation. Further, PDA capping layers were deposited on glass substrates pre-coated with poly(L-lysine) and zwitterionic liposomes. Already thin PDA capping layers render liposome coated surfaces cell adhesive. We experimentally show for the first time, the internalization of a model hydrophobic cargo i.e. fluorescent lipids embedded within the lipid bilayer of liposomes by the cells from the surface. This is evident from the fluorescence exhibited by the cells grown on PDA coatings containing fluorescently labeled liposomes, with the highest fluorescent intensity found in the close proximity of the cell nuclei. The cargo uptake efficiency depends on the thickness of the PDA capping layer and the cell residence time on the coated substrates. Taken together, we demonstrate the first step towards the establishment of a versatile approach using liposomal drug deposits in polymer thin films for surface-mediated drug delivery.
Authors:
Martin E Lynge; Ryosuke Ogaki; Anja Laursen; Jette Lovmand; Duncan Stewart Sutherland; Brigitte Maria Stadler
Related Documents :
25409339 - Treatment of buffalo (bubalus bubalis) donor cells with trichostatin a and 5-aza-2.
18754999 - Imaging amoeboid cancer cell motility in vivo.
22773789 - Cbra is a flavin adenine dinucleotide protein that modifies the escherichia coli outer ...
1706999 - De novo formation of cytokeratin filaments in calf lens cells and cytoplasts after tran...
22308369 - Cell-to-cell movement of plastids in plants.
19214749 - Estimating interfacial tension from the shape histories of cells in compressed aggregat...
3004959 - Permanent expression of p53 in fr 3t3 rat cells but cell cycle-dependent association wi...
11138089 - Fingering in a driven hele-shaw cell
7242089 - The effects of laminin on the growth and differentiation of embryonal carcinoma cells i...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-5-4
Journal Detail:
Title:  ACS applied materials & interfaces     Volume:  -     ISSN:  1944-8244     ISO Abbreviation:  -     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-5-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101504991     Medline TA:  ACS Appl Mater Interfaces     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  DNP by thermal mixing under optimized conditions yields >60,000-fold enhancement of 89Y NMR signal.
Next Document:  The effect of interfacial structure on the transistor properties: Probing the role of surface modifi...