Document Detail


Coated fatty acids alter virulence properties of Salmonella Typhimurium and decrease intestinal colonization of pigs.
MedLine Citation:
PMID:  18583068     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Salmonella Typhimurium infections in pigs are a major source of human foodborne salmonellosis. To reduce the number of infected pigs, acidification of feed or drinking water is a common practice. The aim of the present study was to determine whether some frequently used short- (SCFA) and medium-chain fatty acids (MCFA) are able to alter virulence gene expression and to decrease Salmonella Typhimurium colonization and shedding in pigs using well established and controlled in vitro and in vivo assays. Minimal inhibitory concentrations (MIC) of 4 SCFA (formic acid, acetic acid, propionic acid and butyric acid) and 2 MCFA (caproic and caprylic acid) were determined using 54 porcine Salmonella Typhimurium field strains. MIC values increased at increasing pH-values and were two to eight times lower for MCFA than for SCFA. Expression of virulence gene fimA was significantly lower when bacteria were grown in LB-broth supplemented with sub-MIC concentrations of caproic or caprylic acid (2 mM). Expression of hilA and invasion in porcine intestinal epithelial cells was significantly lower when bacteria were grown in LB-broth containing sub-MIC concentrations of butyric acid or propionic acid (10 mM) and caproic or caprylic acid (2 mM). When given as feed supplement to pigs experimentally infected with Salmonella Typhimurium, coated butyric acid decreased the levels of faecal shedding and intestinal colonization, but had no influence on the colonization of tonsils, spleen and liver. Uncoated fatty acids, however, did not influence fecal shedding, intestinal or tonsillar colonization in pigs. In conclusion, supplementing feed with certain coated fatty acids, such as butyric acid, may help to reduce the Salmonella load in pigs.
Authors:
F Boyen; F Haesebrouck; A Vanparys; J Volf; M Mahu; F Van Immerseel; I Rychlik; J Dewulf; R Ducatelle; F Pasmans
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't     Date:  2008-05-17
Journal Detail:
Title:  Veterinary microbiology     Volume:  132     ISSN:  0378-1135     ISO Abbreviation:  Vet. Microbiol.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2009-01-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705469     Medline TA:  Vet Microbiol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  319-27     Citation Subset:  IM    
Affiliation:
Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. filip.boyen@UGent.be
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MeSH Terms
Descriptor/Qualifier:
Animal Feed / analysis
Animals
Anti-Bacterial Agents / chemistry,  pharmacology
Antigens, Bacterial / genetics,  metabolism
Bacterial Proteins / genetics,  metabolism
Diet / veterinary
Dietary Supplements
Fatty Acids / chemistry*,  pharmacology*
Fimbriae Proteins / genetics,  metabolism
Gene Expression Regulation, Bacterial / drug effects
Intestines / microbiology
Microbial Sensitivity Tests
Salmonella Infections, Animal / microbiology*,  prevention & control
Salmonella typhimurium / metabolism,  pathogenicity*
Swine
Swine Diseases / microbiology,  prevention & control*
Trans-Activators / genetics,  metabolism
Virulence
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Fatty Acids; 0/HilA protein, Salmonella; 0/SEF21 protein, Salmonella enteritidis; 0/Trans-Activators; 147680-16-8/Fimbriae Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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