Document Detail


Coamplification of 12p11 and 12q13 approximately q22 in multiple ring chromosomes in a spindle cell sarcoma resolved by novel multicolor fluorescence in situ hybridization analysis.
MedLine Citation:
PMID:  12547157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An 80-year-old male presented with a lobulated mass in the lower abdominal wall. A diagnosis of an intermediate grade myofibroblastic spindle cell sarcoma was made. Cytogenetic analysis demonstrated a complex karyotype with a der(6), a small marker and five, different in size, ring chromosomes. Fluorescence in situ hybridization (FISH), multiplex FISH, and multicolor banding analysis was used to further delineate this complex karyotype. The der(6) was shown to be a der(18)t(6;18;9;12;18), the marker chromosome was identified as del(17), and the ring chromosomes as r(9) and r(12;18)x4. Amplification of 18 and coamplification of 12p and 12q was detected in the ring and marker chromosomes. No intercellular heterogeneity was observed although a few micronuclei containing chromosome 18 and anaphase bridges, containing chromosome 12 material, the result of bridge-fusion-bridge (BFB) cycles, were observed. Our findings combined with results from others indicate that amplification of chromosomes 12 and 18 as well as BFB phenomena characterize this type of sarcoma.
Authors:
Valia S Lestou; John X O'Connell; Olga Ludkovski; Helena Gosling; David Lesack; Douglas E Horsman
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Publication Detail:
Type:  Case Reports; Journal Article    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  139     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2003-01-27     Completed Date:  2003-02-12     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  44-7     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory Medicine, British Columbia Cancer Agency, r, British Columbia, Vancouve, Canada. vlestou@bccancer.bc.ca
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MeSH Terms
Descriptor/Qualifier:
Chromosome Mapping
Chromosomes, Human, Pair 12*
Chromosomes, Human, Pair 13
Chromosomes, Human, Pair 9
Gene Amplification / genetics
Genetic Markers
Humans
In Situ Hybridization, Fluorescence / methods
Karyotyping
Male
Middle Aged
Ring Chromosomes*
Sarcoma / genetics*
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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