Document Detail


Coaggregation occurs amongst bacteria within and between biofilms in domestic showerheads.
MedLine Citation:
PMID:  23194413     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Showerheads support the development of multi-species biofilms that can be unsightly, produce malodor, and may harbor pathogens. The outer-surface spray-plates of many showerheads support visible biofilms that likely contain a mixture of bacteria from freshwater and potentially from human users. Coaggregation, a mechanism by which genetically distinct bacteria specifically recognize one another, may contribute to the retention and enrichment of different species within these biofilms. The aim of this work was to describe the bacterial composition of outer spray-plate biofilms of three domestic showerheads and to determine the intra- and inter-biofilm coaggregation ability of each culturable isolate. The bacterial composition of the three biofilms was determined by using bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) and by culturing on R2A medium. An average of 31 genera per biofilm were identified using bTEFAP and a total of 30 isolates were cultured. Even though the microbial diversity of each showerhead biofilm differed, every cultured isolate was able to coaggregate with at least one other isolate from the same or different showerhead biofilm. Promiscuous coaggregating isolates belonged to the genera Brevundimonas, Micrococcus, and Lysobacter. This work suggests that coaggregation may be a common feature of showerhead biofilms. Characterization of the mechanisms mediating coaggregation, and the inter-species interactions they facilitate, may allow for novel strategies to inhibit biofilm development.
Authors:
Jay Vornhagen; Michael Stevens; David W McCormick; Scot E Dowd; Joseph N S Eisenberg; Blaise R Boles; Alexander H Rickard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biofouling     Volume:  29     ISSN:  1029-2454     ISO Abbreviation:  Biofouling     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-30     Completed Date:  2013-04-23     Revised Date:  2014-10-19    
Medline Journal Info:
Nlm Unique ID:  9200331     Medline TA:  Biofouling     Country:  England    
Other Details:
Languages:  eng     Pagination:  53-68     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification*,  genetics,  isolation & purification
Bacterial Adhesion
Bacterial Physiological Phenomena*
Bacterial Proteins / genetics
Baths*
Biofilms / growth & development*
Colony Count, Microbial
Fresh Water / microbiology*
Hydrophobic and Hydrophilic Interactions
Microscopy, Confocal
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S / genetics
Sequence Alignment
Sequence Analysis, DNA
Titanium / chemistry
Water Supply
Grant Support
ID/Acronym/Agency:
R01 AI099065/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Ribosomal, 16S; D1JT611TNE/Titanium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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