Document Detail


Co-treatment with hepatocyte growth factor and TGF-beta1 enhances migration of HaCaT cells through NADPH oxidase-dependent ROS generation.
MedLine Citation:
PMID:  20177149     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Wound healing requires re-epithelialization from the wound margin through keratinocyte proliferation and migration, and some growth factors are known to influence this process. In the present study, we found that the co-treatment with hepatocyte growth factor (HGF) and TGF-beta1 resulted in enhanced migration of HaCaT cells compared with either growth factor alone, and that N-acetylcysteine, an antioxidant agent, was the most effective among several inhibitors tested, suggesting the involvement of reactive oxygen species (ROS). Fluorescence-activated cell sorter analysis using 2,7-dichlorofluorescein diacetate (DCF-DA) dye showed an early (30 min) as well as a late (24 h) increase of ROS after scratch, and the increase was more prominent with the growth factor treatment. Diphenyliodonium (DPI), a potent inhibitor of NADPH oxidase, abolished the increase of ROS at 30 min, followed by the inhibition of migration, but not the late time event. More precisely, gene knockdown by shRNA for either Nox-1 or Nox-4 isozyme of gp91phox subunit of NADPH oxidase abolished both the early time ROS production and migration. However, HaCaT cell migration was not enhanced by treatment with H((2))O((2)). Collectively, co-treatment with HGF and TGF-beta1 enhances keratinocyte migration, accompanied with ROS generation through NADPH oxidase, involving Nox-1 and Nox-4 isozymes.
Authors:
Hyun-Ja Nam; Yun-Yeon Park; Gyesoon Yoon; Hyeseong Cho; Jae-Ho Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental & molecular medicine     Volume:  42     ISSN:  2092-6413     ISO Abbreviation:  Exp. Mol. Med.     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-29     Completed Date:  2010-09-20     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  9607880     Medline TA:  Exp Mol Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  270-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Ajou University School of Medicine, Suwon 443-721, Korea.
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MeSH Terms
Descriptor/Qualifier:
Biphenyl Compounds / pharmacology
Cell Movement / drug effects*
Epithelium / drug effects,  enzymology
Gene Knockdown Techniques
Hepatocyte Growth Factor / pharmacology*
Humans
Hydrogen Peroxide / pharmacology
Isoenzymes / metabolism
Keratinocytes / cytology*,  drug effects,  enzymology*
NADPH Oxidase / metabolism*
Onium Compounds / pharmacology
Phosphatidylinositol 3-Kinases / metabolism
Protein Subunits / metabolism
Reactive Oxygen Species / metabolism*
Signal Transduction / drug effects
Time Factors
Transforming Growth Factor beta1 / pharmacology*
Chemical
Reg. No./Substance:
0/Biphenyl Compounds; 0/Isoenzymes; 0/Onium Compounds; 0/Protein Subunits; 0/Reactive Oxygen Species; 0/Transforming Growth Factor beta1; 10182-84-0/diphenyliodonium; 67256-21-7/Hepatocyte Growth Factor; 7722-84-1/Hydrogen Peroxide; EC 1.6.3.-/NOX1 protein, human; EC 1.6.3.-/NOX4 protein, human; EC 1.6.3.1/NADPH Oxidase; EC 2.7.1.-/Phosphatidylinositol 3-Kinases
Comments/Corrections

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