| Co-transcriptional assembly of chemically modified RNA nanoparticles functionalized with siRNAs. | |
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MedLine Citation:
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PMID: 23016824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover, the unpurified transcription mixture can be used for functional ex vivo pilot experiments. |
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Authors:
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Kirill A Afonin; Maria Kireeva; Wade W Grabow; Mikhail Kashlev; Luc Jaeger; Bruce A Shapiro |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural Date: 2012-09-27 |
Journal Detail:
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Title: Nano letters Volume: 12 ISSN: 1530-6992 ISO Abbreviation: Nano Lett. Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-10-10 Completed Date: 2013-02-25 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 101088070 Medline TA: Nano Lett Country: United States |
Other Details:
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Languages: eng Pagination: 5192-5 Citation Subset: IM |
Affiliation:
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Computational RNA Structure Group, Center for Cancer Research Nanobiology Program, NCI, Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, United States. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line, Tumor DNA-Directed RNA Polymerases / metabolism Humans Nanoparticles / administration & dosage, chemistry* Nanotechnology RNA / chemistry*, genetics, metabolism RNA, Small Interfering / chemistry*, genetics*, metabolism Transcription, Genetic Viral Proteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM079604/GM/NIGMS NIH HHS; R01GM-079604/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Small Interfering; 0/Viral Proteins; 63231-63-0/RNA; EC 2.7.7.-/bacteriophage T7 RNA polymerase; EC 2.7.7.6/DNA-Directed RNA Polymerases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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