Document Detail

Co-transcriptional assembly of chemically modified RNA nanoparticles functionalized with siRNAs.
MedLine Citation:
PMID:  23016824     Owner:  NLM     Status:  MEDLINE    
We report a generalized methodology for the one-pot production of chemically modified functional RNA nanoparticles during in vitro transcription with T7 RNA polymerase. The efficiency of incorporation of 2'-fluoro-dNTP in the transcripts by the wild type T7 RNA polymerase dramatically increases in the presence of manganese ions, resulting in a high-yield production of chemically modified RNA nanoparticles functionalized with siRNAs that are resistant to nucleases from human blood serum. Moreover, the unpurified transcription mixture can be used for functional ex vivo pilot experiments.
Kirill A Afonin; Maria Kireeva; Wade W Grabow; Mikhail Kashlev; Luc Jaeger; Bruce A Shapiro
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2012-09-27
Journal Detail:
Title:  Nano letters     Volume:  12     ISSN:  1530-6992     ISO Abbreviation:  Nano Lett.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-10     Completed Date:  2013-02-25     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5192-5     Citation Subset:  IM    
Computational RNA Structure Group, Center for Cancer Research Nanobiology Program, NCI, Frederick National Laboratory for Cancer Research, Frederick, Maryland 21702, United States.
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MeSH Terms
Cell Line, Tumor
DNA-Directed RNA Polymerases / metabolism
Nanoparticles / administration & dosage,  chemistry*
RNA / chemistry*,  genetics,  metabolism
RNA, Small Interfering / chemistry*,  genetics*,  metabolism
Transcription, Genetic
Viral Proteins / metabolism
Grant Support
Reg. No./Substance:
0/RNA, Small Interfering; 0/Viral Proteins; 63231-63-0/RNA; EC 2.7.7.-/bacteriophage T7 RNA polymerase; EC RNA Polymerases

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