| Co-production of 3-hydroxypropionic acid and 1,3-propanediol by Klebseilla pneumoniae expressing aldH under microaerobic conditions. | |
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MedLine Citation:
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PMID: 23201906 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Fed-batch cultures of Klebsiella pneumoniae expressing Escherichia coli aldH were performed under microaerobic conditions to investigate the effects on metabolites production. Increasing the aeration rate enhanced cell growth and 3-hydroxypropionic acid (3-HP) production, but reduced 1,3-propanediol (1,3-PDO) formation. The recombinant strain K. pneumoniae/pUC18kan-aldHec produced 48.9g/L of 3-HP and 25.3g/L of 1,3-PDO with an overall yield of 0.66mol/mol in 28h at an aeration rate of 1.5vvm; however, under fully aerobic condition, no 3-HP and 1,3-PDO were produced due to the repression of dha operon. The flux through the reaction catalyzed by glycerol dehydratase and the split ratio of 1,3-PDO were negatively correlated with the aeration rate, even though the 3-HP level showed a positive trend. This study demonstrated that the relative amounts of 3-HP and 1,3-PDO can be controlled by the aeration rate. |
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Authors:
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Yanna Huang; Zhimin Li; Kazuyuki Shimizu; Qin Ye |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-8 |
Journal Detail:
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Title: Bioresource technology Volume: 128C ISSN: 1873-2976 ISO Abbreviation: Bioresour. Technol. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-12-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9889523 Medline TA: Bioresour Technol Country: - |
Other Details:
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Languages: ENG Pagination: 505-512 Citation Subset: - |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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