Document Detail


Co-localization of prostaglandin F synthase, cyclooxygenase-1 and prostaglandin F receptor in mouse Leydig cells.
MedLine Citation:
PMID:  17674023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to promote better understanding of the physiological roles of prostaglandin F(2alpha) in the mouse testis, we investigated the protein expression and the cellular localization of the enzymes cyclooxygenase and prostaglandin F synthase that are essential for the production of prostaglandin F(2alpha), and the binding site, which is the prostaglandin F(2alpha )receptor (FP). Western blot exhibited the expression of FP protein in wild type mouse testis, and that of prostaglandin F synthase and cyclooxygenase-1 proteins in the both of wild type mouse and FP-deficient mouse testes. The expression of prostaglandin F synthase and cyclooxygenase-1 were detected intensely in Leydig cell-rich fraction, and that of FP was detected equally in Leydig cell-rich fraction and the other fraction. Immunohistochemistry for cyclooxygenase-1 and prostaglandin F synthase demonstrated their co-localization in mouse Leydig cells. Histochemistry for FP demonstrated the localization in Leydig cells and in spermatids of seminiferous tubules. Double histochemical staining confirmed the co-localization of cyclooxygenase-1, prostaglandin F synthase and FP in the Leydig cells. These findings indicate that prostaglandin F(2alpha) may have an effect on the functions of Leyding cells in an autocrine fashion. It implies that prostaglandin F synthase and FP are involved in the control of testosterone release from Leydig cells and in spermatogenesis via the local pathway and the hypothalamo-hypophysial-testis pathway, and affect the testicular function.
Authors:
Toshiko Suzuki-Yamamoto; Yukihiko Sugimoto; Atsushi Ichikawa; Kazunori Ishimura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-03
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  128     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-09-17     Completed Date:  2008-01-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  317-22     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Cell Biology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto, Tokushima, 770-8503, Japan. toshiko@basic.med.tokushima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cyclooxygenase 1 / metabolism*
Hydroxyprostaglandin Dehydrogenases / metabolism*
Immunohistochemistry
Leydig Cells / metabolism*
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Prostaglandin / deficiency,  genetics,  metabolism*
Testis / metabolism
Chemical
Reg. No./Substance:
0/Receptors, Prostaglandin; 0/prostaglandin F2alpha receptor; EC 1.1.1.-/Hydroxyprostaglandin Dehydrogenases; EC 1.1.1.188/prostaglandin-F synthase; EC 1.14.99.1/Cyclooxygenase 1

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