Document Detail

Co-localization of neurophysin- and enkephalin-like immunoreactivity in cat pituitary.
MedLine Citation:
PMID:  6167969     Owner:  NLM     Status:  MEDLINE    
Enkephalin and neurophysin immunoreactivity have been co-localized in terminals of frozen-dried cat posterior pituitary, using two methods of immunocytochemistry--the protein A-gold procedure and the PAP method. Absorption controls show reduced staining in all cases. Intermediate lobe cells are negative using the enkephalin and neurophysin antisera, but with alpha-MSH antiserum, posterior lobe terminals are negative and intermediate lobe cells are positive. The data are compatible with the hypothesis that inhibition of release of oxytocin and vasopressin by the pituitary opioid system is accomplished by an autoregulatory mechanism in which the release of enkephalin with oxytocin or vasopressin serves to inhibit release of the neurohormones.
H D Coulter; R P Elde; S L Unverzagt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Peptides     Volume:  2 Suppl 1     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  1981  
Date Detail:
Created Date:  1981-10-25     Completed Date:  1981-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  51-5     Citation Subset:  IM    
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MeSH Terms
Endorphins / analysis*
Enkephalins / analysis*
Neurophysins / analysis*
Pituitary Gland / analysis*,  cytology
Staining and Labeling
Grant Support
Reg. No./Substance:
0/Endorphins; 0/Enkephalins; 0/Neurophysins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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