| Co-localization of a kallikrein-like serine protease (arginine esterase A) and atrial natriuretic peptide in rat atrium. | |
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MedLine Citation:
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PMID: 2531183 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Atrial natriuretic peptide (ANP) is stored in atrial granules primarily as a larger molecular weight precursor (pro-ANP), which is believed to be rapidly converted to an active peptide of 28 amino acids during or shortly after secretion. A tissue kallikrein-like serine protease has been suggested as a potential processing enzyme. In the present immunocytochemical study, using specific monoclonal antibodies, we found that esterase A, a kallikrein-like serine protease, was demonstrable in rat atrial myocytes and in ventricular myocytes, and was capable of cleaving pro-ANP to yield a low molecular weight product. Using colloidal gold immunocytochemistry at the electron microscopic level, we have found esterase A in atrial myocytes, both in granules and in another subcellular site that corresponds to sarcoplasmic reticulum. Double-label electron microscopic immunocytochemical results indicated that esterase A can co-localize with ANP in granules of atrial myocytes. |
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Authors:
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J A Simson; M G Currie; L Chao; J Chao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society Volume: 37 ISSN: 0022-1554 ISO Abbreviation: J. Histochem. Cytochem. Publication Date: 1989 Dec |
Date Detail:
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Created Date: 1989-12-22 Completed Date: 1989-12-22 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815334 Medline TA: J Histochem Cytochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1913-7 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Medical University of South Carolina, Charleston 29425. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atrial Natriuretic Factor / metabolism* Carboxylic Ester Hydrolases / metabolism* Cytoplasmic Granules / metabolism Heart Atria / metabolism* Immunohistochemistry Microscopy, Electron Protein Processing, Post-Translational Rats |
| Grant Support | |
ID/Acronym/Agency:
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HL 29397/HL/NHLBI NIH HHS; HL 33552/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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85637-73-6/Atrial Natriuretic Factor; EC 3.1.-/arginine esterase; EC 3.1.1.-/Carboxylic Ester Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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