Document Detail

Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat.
MedLine Citation:
PMID:  20691166     Owner:  NLM     Status:  MEDLINE    
We have reported a highly cooperative interaction between leptin and thyrotropin releasing hormone (TRH) in the hindbrain to generate thermogenic responses (Hermann et al., 2006) (Rogers et al., 2009). Identifying the locus in the hindbrain where leptin and TRH act synergistically to increase thermogenesis will be necessary before we can determine the mechanism(s) by which this interaction occurs. Here, we performed heat-induced epitope recovery techniques and in situ hybridization to determine if neurons or afferent fibers in the hindbrain possess both TRH type 1 receptor and long-form leptin receptor [TRHR1; LepRb, respectively]. LepRb receptors were highly expressed in the solitary nucleus [NST], dorsal motor nucleus of the vagus [DMN] and catecholaminergic neurons of the ventrolateral medulla [VLM]. All neurons that contained LepRb also contained TRHR1. Fibers in the NST and the raphe pallidus [RP] and obscurrus [RO] that possess LepRb receptors were phenotypically identified as glutamatergic type 2 fibers (vglut2). Fibers in the NST and RP that possess TRHR1 receptors were phenotypically identified as serotonergic [i.e., immunopositive for the serotonin transporter; SERT]. Co-localization of LepRb and TRHR1 was not observed on individual fibers in the hindbrain but these two fiber types co-mingle in these nuclei. These anatomical arrangements may provide a basis for the synergy between leptin and TRH to increase thermogenesis.
Maria J Barnes; Richard C Rogers; Montina J Van Meter; Gerlinda E Hermann
Related Documents :
3031716 - Effect of receptor blockers (methysergide, propranolol, phentolamine, yohimbine and pra...
3036246 - Transferrin and ferritin endocytosis and recycling in guinea-pig reticulocytes.
2365686 - Nonacylated human transferrin receptors are rapidly internalized and mediate iron uptake.
12038596 - Serum transferrin receptor assays and their application.
10696996 - Angiotensin ii receptor antagonists.
18849166 - The identification of neurotensin nts1 receptor partial agonists through a ligand-based...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-08-04
Journal Detail:
Title:  Brain research     Volume:  1355     ISSN:  1872-6240     ISO Abbreviation:  Brain Res.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-13     Completed Date:  2011-06-20     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  70-85     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Medulla Oblongata / chemistry,  cytology,  metabolism*
Mice, Inbred C57BL
Mice, Obese
Neurons / chemistry,  cytology,  metabolism*
Raphe Nuclei / physiology
Rats, Long-Evans
Receptors, Leptin / genetics,  metabolism*
Receptors, Thyrotropin-Releasing Hormone / chemistry,  genetics,  metabolism*
Reticular Formation / cytology,  metabolism
Rhombencephalon / chemistry,  cytology,  metabolism*
Solitary Nucleus / cytology,  metabolism
Vagus Nerve / cytology,  metabolism
Grant Support
Reg. No./Substance:
0/Receptors, Leptin; 0/Receptors, Thyrotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Eicosapentaenoic acid improves hepatic steatosis independent of PPARalpha activation through inhibit...
Next Document:  Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz elec...